Palankar Raghavendra, Pinchasik Bat-El, Schmidt Stephan, De Geest Bruno G, Fery Andreas, Möhwald Helmuth, Skirtach André G, Delcea Mihaela
ZIK HIKE, Nanostructure Group, Ernst-Moritz-Arndt-Universität Greifswald, Greifswald 17489, Germany.
J Mater Chem B. 2013 Feb 28;1(8):1175-1181. doi: 10.1039/c2tb00319h. Epub 2013 Jan 10.
Developing carriers comprised of biomaterials and capable of withstanding significant mechanical pressures, structural deformations and at the same time delivering biomolecules is of high interest for drug delivery. Using colloidal probe AFM combined with quantitative fluorescence microscopy, we investigated mechanical properties and release from CaCO-templated polymeric capsules made of biodegradable polymers. The mechanical and release properties of these capsules were studied in comparison with those of CaCO-templated capsules composed of synthetic polymers. Furthermore, we assessed the influence of the number of polyelectrolyte LbL layers on the mechanical properties and release from biodegradable capsules. Mechanical deformation of capsules was studied upon intracellular uptake of these capsules. Based on these results, we discuss implications for the design of microcapsules.
开发由生物材料组成且能够承受显著机械压力、结构变形并同时递送生物分子的载体，对于药物递送具有高度的吸引力。我们使用胶体探针原子力显微镜结合定量荧光显微镜，研究了由可生物降解聚合物制成的碳酸钙模板化聚合物胶囊的机械性能和释放情况。将这些胶囊的机械性能和释放性能与由合成聚合物组成的碳酸钙模板化胶囊进行了比较研究。此外，我们评估了聚电解质层层组装层数对可生物降解胶囊的机械性能和释放的影响。在这些胶囊被细胞摄取后，研究了胶囊的机械变形情况。基于这些结果，我们讨论了对微胶囊设计的启示。
