Maji Swarup Kumar, Dutta Amit Kumar, Bhadu Gopala Ram, Paul Parimal, Mondal Anup, Adhikary Bibhutosh
Department of Chemistry, Bengal Engineering and Science University, Shibpur, Howrah 711 103, West Bengal, India.
J Mater Chem B. 2013 Sep 7;1(33):4127-4134. doi: 10.1039/c3tb20846j. Epub 2013 Jul 8.
A facile, greener and template free route has been developed to produce cuprous sulfide (CuS) nanoplates (NPs) with average diameters of 70-150 nm, via one step solvothermal decomposition of a single-source precursor (SSP) Cu(ACDC) [ACDC = 2-aminocyclopentene-1-dithiocarboxylate] in the presence of ethylenediamine (EN) and triethylenetetramine (TETA) as structure orienting agents. The precursor complex and nanomaterials were thoroughly characterized by several common techniques and measurements, which give the composition and characteristics of the materials. Amperometric biosensors for hydrogen peroxide (HO) and glucose have been constructed by immobilizing the synthesized CuS NPs in glutaraldehyde on a glassy carbon (GC) electrode using a direct drop-coating method. The proposed sensor has displayed faster response, high and reproducible sensitivity (64.27 μA mM) with linear range of 10 μM to 3.75 mM, towards the electrochemical biosensing of HO at -0.35 V (vs. Ag/AgCl). The sensor also showed high and reproducible sensitivity (61.67 μA mM) towards glucose determination with linear range of 10 μM to 3.1 mM. The anti-inference ability of electroactive molecules and favorable stability are some of the advantages of the proposed sensor. Finally, using the sensor we have determined the glucose concentration in a human blood serum sample. The results strongly demonstrate the usefulness of CuS NPs for biosensor design and other biological applications.
通过一步溶剂热分解单源前驱体（SSP）Cu(ACDC) [ACDC = 2-氨基环戊烯-1-二硫代羧酸盐]，在乙二胺（EN）和三亚乙基四胺（TETA）作为结构导向剂的存在下，开发了一种简便、绿色且无模板的路线来制备平均直径为70-150 nm的硫化亚铜（CuS）纳米片（NPs）。通过几种常用技术和测量对前驱体配合物和纳米材料进行了全面表征，从而得出材料的组成和特性。采用直接滴涂法，将合成的CuS NPs固定在玻碳（GC）电极上的戊二醛中，构建了用于过氧化氢（HO）和葡萄糖的安培型生物传感器。所提出的传感器在-0.35 V（相对于Ag/AgCl）下对HO的电化学生物传感显示出更快的响应、高且可重复的灵敏度（64.27 μA mM），线性范围为10 μM至3.75 mM。该传感器对葡萄糖测定也显示出高且可重复的灵敏度（61.67 μA mM），线性范围为10 μM至3.1 mM。所提出的传感器的优点包括电活性分子的抗干扰能力和良好的稳定性。最后，使用该传感器测定了人血清样品中的葡萄糖浓度。结果有力地证明了CuS NPs在生物传感器设计和其他生物应用中的实用性。
