Wu Chengtie, Fan Wei, Chang Jiang
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People's Republic of China.
J Mater Chem B. 2013 Jun 7;1(21):2710-2718. doi: 10.1039/c3tb20275e. Epub 2013 Apr 22.
Controllable drug delivery is one of the important ways for the therapy of bone cancer. Conventional mesoporous silica nano-particles may lack dual properties for combining controllable delivery of anti-cancer drugs and bone-forming bioactivity for bone cancer therapy. The aim of this study is to synthesize mesoporous bioactive glass (MBG) nanospheres with combined dual functions of bioactivity and controlled drug delivery, and to further investigate their delivery property of anti-cancer drugs as well as the functional effect on bone-cancer cells. MBG nanospheres with spherical morphology and internal mesoporous microstructures were successfully prepared by a facile hydrothermal method. The prepared MBG nanospheres possess high specific surface area and mesopore volume (443 m g, 0.57 cm g) as well as uniform mesopore size distribution (2.9 nm). The MBG nanospheres demonstrate excellent bioactivity by inducing apatite mineralization in simulated body fluids. An anti-cancer drug, doxorubicin hydrochloride (DOX), was successfully loaded in the MBG nanospheres with a distinctively high loading efficiency of around 90%. The loading amount of DOX can be effectively controlled by adjusting the initial drug-loading concentrations. MBG nanospheres can maintain a sustained release of DOX, and their release kinetics can be controlled by varying the pH microenvironment and initial drug-loading concentrations. In addition, the prepared MBG nanospheres showed obvious degradation by releasing Ca and SiO ions in PBS. Furthermore, the delivery of DOX from MBG nanospheres into cell culture environment shows a significant inhibitory effect on the viability of osteosarcoma cells with the increase of interaction time. The prepared MBG nanospheres have high specific surface area and mesopore volume, excellent apatite-mineralization ability, distinct degradability, high DOX-loading efficiency and controllable DOX release as well as anti-cancer functions. These unique characteristics suggest that the obtained MBG nanospheres may be used for the therapy of bone cancer.
可控药物递送是骨癌治疗的重要途径之一。传统的介孔二氧化硅纳米颗粒可能缺乏将抗癌药物的可控递送与骨形成生物活性相结合以用于骨癌治疗的双重特性。本研究的目的是合成具有生物活性和可控药物递送双重功能的介孔生物活性玻璃（MBG）纳米球，并进一步研究其抗癌药物递送特性以及对骨癌细胞的功能作用。通过简便的水热法成功制备了具有球形形态和内部介孔微结构的MBG纳米球。所制备的MBG纳米球具有高比表面积和介孔体积（443 m²/g，0.57 cm³/g）以及均匀的介孔尺寸分布（2.9 nm）。MBG纳米球通过在模拟体液中诱导磷灰石矿化表现出优异的生物活性。一种抗癌药物盐酸阿霉素（DOX）成功负载到MBG纳米球中，负载效率高达约90%。通过调节初始载药浓度可以有效控制DOX的负载量。MBG纳米球可以维持DOX的持续释放，并且其释放动力学可以通过改变pH微环境和初始载药浓度来控制。此外，所制备的MBG纳米球在PBS中通过释放Ca和SiO离子表现出明显的降解。此外，DOX从MBG纳米球向细胞培养环境的递送随着相互作用时间的增加对骨肉瘤细胞的活力显示出显著的抑制作用。所制备的MBG纳米球具有高比表面积和介孔体积、优异的磷灰石矿化能力、明显的降解性、高DOX负载效率和可控的DOX释放以及抗癌功能。这些独特的特性表明所获得的MBG纳米球可用于骨癌治疗。
