Chen Ya-Yi, Chiou Yuh-Jing, Chang Pei-Jung, Chang Wei-Min, Yeh Yu-Cheng, Chen Chin-Yi, Chang Yu-Kang, Lin Chung-Kwei
Doctoral Program in Medical Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
Department of Stomatology, Tung's Taichung Metro Harbor Hospital, Taichung 435, Taiwan.
Polymers (Basel). 2024 Oct 4;16(19):2811. doi: 10.3390/polym16192811.
Preparing electrospun fibers by applying a potential difference between a polymeric solution and a contacting substrate is increasingly attracting attention in tissue engineering applications. Among the numerous polymers, polycaprolactone (PCL) bioscaffold has been widely investigated due to its biocompatibility and biodegradability. Bioactive powder can be added to further improve its performance. In the present study, bioactive glass powder modified by adding 0-6 wt.% antibacterial zinc element (coded as ZBG) was prepared through the sol-gel process. Furthermore, PCL bioscaffolds with various ZBG additions were prepared using the electrospinning technique. The zinc-doped bioactive glass powder and electrospun PCL/ZBG bioscaffolds were evaluated using scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy to determine their structural properties. Additionally, in vitro bioactivity, biocompatibility and antibacterial performance were investigated. Experimental results showed that sol-gelled ZBG powder possessed superior bioactivity and 0.8 g ZBG was the optimal addition to prepare PCL/ZBG bioscaffolds with. All the electrospun PCL/ZBG bioscaffolds were biocompatible and their antibacterial performance against two strains (SA133 and Newman) improved with increasing zinc concentration. Electrospun PCL/ZBG bioscaffolds exhibited excellent bioactivity and have great potential for biomedical application.
通过在聚合物溶液和接触基底之间施加电势差来制备电纺纤维，在组织工程应用中越来越受到关注。在众多聚合物中，聚己内酯（PCL）生物支架因其生物相容性和生物降解性而受到广泛研究。可以添加生物活性粉末以进一步改善其性能。在本研究中，通过溶胶-凝胶法制备了添加0-6 wt.%抗菌锌元素改性的生物活性玻璃粉末（编码为ZBG）。此外，采用电纺丝技术制备了添加不同量ZBG的PCL生物支架。使用扫描电子显微镜、X射线衍射和傅里叶变换红外光谱对掺锌生物活性玻璃粉末和电纺PCL/ZBG生物支架进行了评估，以确定它们的结构特性。此外，还研究了其体外生物活性、生物相容性和抗菌性能。实验结果表明，溶胶凝胶法制备的ZBG粉末具有优异的生物活性，0.8 g ZBG是制备PCL/ZBG生物支架的最佳添加量。所有电纺PCL/ZBG生物支架均具有生物相容性，并且它们对两种菌株（SA133和Newman）的抗菌性能随着锌浓度的增加而提高。电纺PCL/ZBG生物支架表现出优异的生物活性，在生物医学应用方面具有巨大潜力。
