Li Hao, Kong Weiqian, Liu Juan, Yang Manman, Huang Hui, Liu Yang, Kang Zhenhui
Institute of Functional Nano& Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, and Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu, China.
J Mater Chem B. 2014 Sep 14;2(34):5652-5658. doi: 10.1039/c4tb00705k. Epub 2014 Jul 24.
Enzyme engineering for improved catalysis has wide implications. Here, we report the modulation of enzyme (porcine pancreatic lipase, PPL) catalytic activity in the presence of carbon quantum dots (CQDs) by visible light. Upon visible light irradiation, the activity of PPL/CQDs increased to 10% higher than that of free PPL, whereas without a light source, the activity of PPL/CQDs decreased to 30% lower than that of free PPL. Based on Michaelis-Menten kinetics, CQDs were confirmed to play an important role as a non-competitive inhibitor. Our results present a potential new method for developing hyper-catalytic hybrid enzymes for bio-catalytic applications.
用于改善催化作用的酶工程具有广泛的意义。在此，我们报道了在可见光存在下通过碳量子点（CQDs）对酶（猪胰脂肪酶，PPL）催化活性的调节。在可见光照射下，PPL/CQDs的活性比游离PPL的活性提高了10%，而在没有光源的情况下，PPL/CQDs的活性比游离PPL的活性降低了30%。基于米氏动力学，证实CQDs作为非竞争性抑制剂发挥重要作用。我们的结果为开发用于生物催化应用的超催化杂合酶提供了一种潜在的新方法。
