Fraix Aurore, Manet Ilse, Ballestri Marco, Guerrini Andrea, Dambruoso Paolo, Sotgiu Giovanna, Varchi Greta, Camerin Monica, Coppellotti Olimpia, Sortino Salvatore
Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, I-95125 Catania, Italy.
J Mater Chem B. 2015 Apr 21;3(15):3001-3010. doi: 10.1039/c5tb00234f. Epub 2015 Mar 4.
We have developed a multifunctional polymer-based nanoplatform for bimodal cancer phototherapy. It was achieved by electrostatic entangling of two anionic photoactivable components, a commercial porphyrin and a tailored nitro-aniline derivative, within the cationic shell of polymeric nanoparticles (NPs) based on polymethyl methacrylate. The combination of steady-state and time-resolved spectroscopic and photochemical techniques shows that the two photoresponsive agents do not interfere with each other while being in close proximity in the same polymeric scaffold and can thus operate in parallel under the exclusive control of light stimuli. Specifically, visible light triggers satisfactory red fluorescence emission and generation of singlet oxygen (O) from one component and release of nitric oxide (NO) from the other. Fluorescence microscopy analysis provides unambiguous evidence for the internalization of the NPs within B78H1 melanoma cells, where they induce amplified mortality due to a combinatory effect of the two photogenerated cytotoxic species.
我们开发了一种用于双模态癌症光疗的多功能聚合物基纳米平台。它是通过将两种阴离子光活性成分，一种商用卟啉和一种定制的硝基苯胺衍生物，在基于聚甲基丙烯酸甲酯的聚合物纳米颗粒（NPs）的阳离子壳层内进行静电缠结而实现的。稳态和时间分辨光谱及光化学技术的结合表明，这两种光响应剂在同一聚合物支架中紧密相邻时不会相互干扰，因此可以在光刺激的单独控制下并行发挥作用。具体而言，可见光触发一种成分产生令人满意的红色荧光发射并生成单线态氧（O），同时触发另一种成分释放一氧化氮（NO）。荧光显微镜分析为NPs在B78H1黑色素瘤细胞内的内化提供了明确证据，在这些细胞中，由于两种光生细胞毒性物质的联合作用，它们会导致细胞死亡率增加。
