An electrochemical bifunctional sensor for the detection of nitrite and hydrogen peroxide based on layer-by-layer multilayer films of cationic phthalocyanine cobalt(ii) and carbon nanotubes.

As vital biological mediators, the accurate detection of nitrite (NO ) and hydrogen peroxide (HO) is desirable for clinical monitoring and diagnosis. Herein, cationic 2,9,16,23-tetra[4-(N-methyl)pyridinyloxy]phthalocyanine cobalt(ii) ([TMPyPcCo]) and acid-treated multiwalled carbon nanotubes (aCNTs) were alternately self-assembled on the glassy carbon electrode (GCE) by means of the electrostatic interaction, leading to a 3D loose and interconnected assembly of [TMPyPcCo/aCNTs] multilayer films. In the [TMPyPcCo/aCNTs] films, [TMPyPcCo] is anchored onto the surface of aCNTs without any inert polymer binders, which is beneficial to expose more active sites for electrocatalysis. The effective combination of [TMPyPcCo] and aCNTs brings many advantages in electrochemical detection, involving the fast oriented transmission of charges, permeable channels for ion adsorption and transport, and more sensing sites, thus the [TMPyPcCo/aCNTs] films display excellent electrochemical sensitivity towards both NO and HO. The responses of NO and HO vary linearly with respect to the concentration from 5 µM to 30 mM and 10 µM to 9 mM. Furthermore, the superior cycling stability, reproducibility, and selectivity make [TMPyPcCo/aCNTs] films suitable for the real samples.