Sharma Vinay, Saini Anoop Kumar, Mobin Shaikh M
Centre for Biosciences and Bio-Medical Engineering, Indian Institute of Technology Indore, Simrol, Indore-452020, India.
J Mater Chem B. 2016 Apr 14;4(14):2466-2476. doi: 10.1039/c6tb00238b. Epub 2016 Mar 21.
Carbon based nanomaterials are emerging as a desirable alternative to semiconducting quantum dots due to their unique optical properties and biocompatibility. The present study demonstrates the design and synthesis of highly fluorescent carbon nanoparticles (CNPs). The CNPs are investigated for their biocompatibility and henceforth successfully employed as promising multicolor bioimaging probes in A375 and DU145 cell lines. Furthermore, a "turn off" mode has been established for the detection of noble metal palladium (Pd) and heavy metal mercury (Hg) by quenching the fluorescence of CNPs. The CNP sensor responded to the detection of Pd (5-100 μM) and Hg (1-18 μM) in a wide range with the limit of detection (LOD) of 58 nM for Pd and 100 nM for Hg. The CNP sensor was employed for the detection of Pd and Hg in real water samples and detection of leftover palladium catalysts in a model reaction system. Also, the CNPs were successfully employed as intracellular mercury and palladium sensors using confocal microscopy.
碳基纳米材料因其独特的光学性质和生物相容性，正成为半导体量子点的理想替代品。本研究展示了高荧光碳纳米颗粒（CNPs）的设计与合成。对CNPs的生物相容性进行了研究，并随后成功将其用作A375和DU145细胞系中有前景的多色生物成像探针。此外，通过淬灭CNPs的荧光，建立了一种“关闭”模式用于检测贵金属钯（Pd）和重金属汞（Hg）。CNP传感器在很宽的范围内对Pd（5 - 100 μM）和Hg（1 - 18 μM）的检测有响应，Pd的检测限（LOD）为58 nM，Hg的检测限为100 nM。该CNP传感器用于实际水样中Pd和Hg的检测以及模型反应体系中残留钯催化剂的检测。此外，利用共聚焦显微镜，CNPs成功用作细胞内汞和钯传感器。
