Spinato Cinzia, Giust Davide, Vacchi Isabella Anna, Ménard-Moyon Cécilia, Kostarelos Kostas, Bianco Alberto
CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Thérapeutique, 67000 Strasbourg, France.
J Mater Chem B. 2016 Jan 21;4(3):431-441. doi: 10.1039/c5tb02088c. Epub 2015 Dec 16.
In this work, we have investigated the preparation of amino-functionalised multi-walled carbon nanotubes (MWCNTs) as potential carriers for the delivery of siRNA. Several studies have shown promising results exploiting functionalised CNTs for the delivery of genetic material in vitro and in vivo. Our groups have previously observed that the type of surface functionalisation used to modify oxidised MWCNTs (oxMWCNTs) can lead to significant differences in nanotube cellular uptake and delivery capability. In those studies, amino-functionalised CNTs were obtained by cycloaddition reactions. Here, we focused on the direct conversion of the carboxylic groups present on oxMWCNTs into amines, and we attempted different synthetic strategies in order to directly tether the amines onto the CNTs, without extending the lateral chain. The functionalised material was characterised by X-ray photoelectron spectroscopy, Fourier transform infra-red spectroscopy and transmission electron microscopy, and the most water-dispersible CNTs were selected for siRNA complexation and cellular uptake studies. The aminated conjugates are demonstrated to be promising vectors to achieve intracellular transport of genetic information.
在本研究中，我们研究了氨基功能化多壁碳纳米管（MWCNTs）作为小干扰RNA（siRNA）递送潜在载体的制备方法。多项研究表明，利用功能化碳纳米管在体外和体内递送遗传物质取得了有前景的结果。我们团队之前观察到，用于修饰氧化多壁碳纳米管（oxMWCNTs）的表面功能化类型会导致纳米管细胞摄取和递送能力的显著差异。在那些研究中，氨基功能化碳纳米管是通过环加成反应获得的。在此，我们专注于将oxMWCNTs上存在的羧基直接转化为胺，并尝试了不同的合成策略，以便在不延长侧链的情况下将胺直接连接到碳纳米管上。通过X射线光电子能谱、傅里叶变换红外光谱和透射电子显微镜对功能化材料进行了表征，并选择了最具水分散性的数据纳米管进行siRNA复合和细胞摄取研究。结果表明，胺化缀合物是实现遗传信息细胞内运输的有前景的载体。
