An Xiangyang, He Xiwen, Chen Langxing, Zhang Yukui
Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
J Mater Chem B. 2016 Sep 28;4(36):6125-6133. doi: 10.1039/c6tb01489e. Epub 2016 Sep 1.
Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. In this work, a facile and efficient method was developed to synthesize boronic acid polymer brushes immobilized on magnetic graphene oxide via surface initiated atom transfer radical polymerization (SI-ATRP) for the selective enrichment of glycoproteins from complex biological samples. The magnetic graphene oxide (GO@FeO) nanocomposites were prepared by a solvothermal reaction, providing an ultrahigh surface area and allowing fast separation. Through the self-assembly procedure, the pyrene-based initiators (GO@Br) of SI-ATRP were easily functionalized on the GO sheet via noncovalent π-π interaction between pyrene and GO. Finally, the well-defined and high density poly(4-vinylphenylboronic acid) brushes (GO@PVPBA) via SI-ATRP were successfully fabricated. The morphology and structure of GO@FeO, GO@Br, and GO@PVPBA nanocomposites were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), vibrating sample magnetometry (VSM), Fourier transform-infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The selective recognition capability of GO@PVPBA nanocomposites was demonstrated by the selective enrichment of glycoproteins from a complex system consisting of standard proteins ovalbumin (OVA), transferrin (Trf), bovine serum albumin (BSA), and lysozyme (Lyz). Furthermore, the GO@PVPBA nanocomposite also exhibited a high binding capacity up to 514.8 and 445.9 mg g for OVA and Trf, respectively, and was applied to capture directly glycoproteins from the egg white samples.
生物医学科学，尤其是生物标志物研究，需要高效的糖蛋白富集平台。在这项工作中，开发了一种简便高效的方法，通过表面引发原子转移自由基聚合（SI-ATRP）合成固定在磁性氧化石墨烯上的硼酸聚合物刷，用于从复杂生物样品中选择性富集糖蛋白。通过溶剂热反应制备了磁性氧化石墨烯（GO@FeO）纳米复合材料，其具有超高的表面积并能实现快速分离。通过自组装过程，SI-ATRP的芘基引发剂（GO@Br）通过芘与GO之间的非共价π-π相互作用轻松地在GO片上功能化。最后，成功制备了通过SI-ATRP定义明确且高密度的聚（4-乙烯基苯硼酸）刷（GO@PVPBA）。通过透射电子显微镜（TEM）、X射线衍射（XRD）、振动样品磁强计（VSM）、傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）对GO@FeO、GO@Br和GO@PVPBA纳米复合材料的形态和结构进行了表征。通过从由标准蛋白卵清蛋白（OVA）、转铁蛋白（Trf）、牛血清白蛋白（BSA）和溶菌酶（Lyz）组成的复杂系统中选择性富集糖蛋白，证明了GO@PVPBA纳米复合材料的选择性识别能力。此外，GO@PVPBA纳米复合材料对OVA和Trf的结合容量分别高达514.8和445.9 mg g，并且被应用于直接从蛋清样品中捕获糖蛋白。
