Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, People's Republic of China.
Analyst. 2019 Jan 14;144(2):641-648. doi: 10.1039/c8an01704b.
Biomedical applications and biomarkers for early clinical diagnostics and the treatment of diseases demand efficient and selective enrichment platforms for glycoproteins. Thus, we herein report a facile and general approach for the preparation of boronic acid-functionalized magnetic nanoparticles for the selective enrichment of glycoproteins. More specifically, Fe3O4 magnetic nanoparticles were initially prepared via a solvothermal reaction, and core-shell-structured Fe3O4@SiO2 nanoparticles were obtained according to a sol-gel process. Subsequently, the Fe3O4@SiO2 surfaces were modified using 4-formylphenylboronic acid to allow the formation of strong yet reversible covalent bonds between boronic acid (BA) and the cis-1,2-diol groups of glycoproteins. The morphology and structure of the Fe3O4, Fe3O4@SiO2, and Fe3O4@SiO2-BA nanoparticles were characterized by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and vibrating sample magnetometry, thereby confirming their successful preparation. The obtained BA-modified Fe3O4@SiO2 magnetic nanoparticles were applied in the attempted enrichment of two glycoproteins (ovalbumin (OVA) and transferrin (TRF)) and two non-glycoproteins (bovine serum albumin (BSA) and cytochrome c (Cyt C)). The results confirmed a significant difference in affinity between glycoproteins and non-glycoproteins. In addition, the recognition capability of the Fe3O4@SiO2-BA nanoparticles was demonstrated by the selective enrichment of glycoproteins from a complex system containing both glycoproteins (i.e., TRF) and non-glycoproteins (i.e., Cyt C).
用于早期临床诊断和疾病治疗的生物医学应用和生物标志物需要高效和选择性的糖蛋白富集平台。因此，我们在此报告了一种简便通用的方法，用于制备硼酸功能化的磁性纳米粒子，以选择性富集糖蛋白。具体来说，首先通过溶剂热反应制备了 Fe3O4 磁性纳米粒子，并根据溶胶-凝胶法获得了核壳结构的 Fe3O4@SiO2 纳米粒子。随后，使用 4-醛基苯硼酸对 Fe3O4@SiO2 表面进行修饰，以允许硼酸（BA）与糖蛋白的顺式 1,2-二醇基团之间形成强而可逆的共价键。通过扫描电子显微镜、X 射线衍射、傅里叶变换红外光谱、X 射线光电子能谱和振动样品磁强计对 Fe3O4、Fe3O4@SiO2 和 Fe3O4@SiO2-BA 纳米粒子的形貌和结构进行了表征，从而证实了它们的成功制备。所得 BA 修饰的 Fe3O4@SiO2 磁性纳米粒子应用于两种糖蛋白（卵清蛋白（OVA）和转铁蛋白（TRF））和两种非糖蛋白（牛血清白蛋白（BSA）和细胞色素 c（Cyt C））的尝试性富集。结果证实了糖蛋白和非糖蛋白之间的亲和力存在显著差异。此外，通过从含有糖蛋白（即 TRF）和非糖蛋白（即 Cyt C）的复杂体系中选择性富集糖蛋白，证明了 Fe3O4@SiO2-BA 纳米粒子的识别能力。
