Ren Mingguang, Deng Beibei, Zhou Kai, Wang Jian-Yong, Kong Xiuqi, Lin Weiying
Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China.
J Mater Chem B. 2017 Mar 14;5(10):1954-1961. doi: 10.1039/c6tb03388a. Epub 2017 Feb 20.
Nitroxyl plays crucial roles in many biological pathways and can serve as a potent therapeutic agent for the treatment of heart failure. Recent studies suggest that HNO may be produced in mitochondria and the HNO formed might have functional consequences for mitochondrial activity. However, in order to study the function of HNO in mitochondria, a suitable research method is needed. Herein, through rational design, we synthesized a new mitochondria-targeted fluorescent nitroxyl probe (Mito-HNO). The developed probe was highly selective toward HNO over other reactive nitrogen species and reducing species. In addition, the probe Mito-HNO was rapidly responsive and suitable for visualization of HNO in mitochondria in living cells. The probe is expected to be employed in further revealing the biological function of HNO in subcellular mitochondria.
硝酰在许多生物途径中发挥着关键作用，并且可以作为治疗心力衰竭的有效治疗剂。最近的研究表明，线粒体中可能产生HNO，并且形成的HNO可能对线粒体活性产生功能性影响。然而，为了研究HNO在线粒体中的功能，需要一种合适的研究方法。在此，通过合理设计，我们合成了一种新型的线粒体靶向荧光硝酰探针（Mito-HNO）。所开发的探针对HNO的选择性高于其他活性氮物种和还原物种。此外，探针Mito-HNO响应迅速，适用于活细胞中线粒体中HNO的可视化。该探针有望用于进一步揭示HNO在亚细胞线粒体中的生物学功能。
