Zhang Zulei, Zhang Xingdi, Niu Dechao, Li Yongsheng, Shi Jianlin
Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
J Mater Chem B. 2017 Jun 14;5(22):4214-4220. doi: 10.1039/c7tb00886d. Epub 2017 May 18.
Natural antibodies are used widely for various applications such as in biomedical analysis, protein separation, and targeted-drug delivery, but they suffer from high cost and low stability. In this study, we developed a facile approach for the construction of antibody-like binding sites in a porous silica solid for efficient separation of bovine serum albumin (BSA) based on large-pore silica particles (LPSPs). This was accomplished by grafting two types of organosilane monomers, 3-aminopropyltriethoxylsilane (APTES) and octyltrimethoxysilane (OTMS), to provide hydrogen bonds or hydrophobic interactions with BSA through molecular imprinting technology. The resulting molecularly imprinted, large-pore silica particles (MI-LPSPs) were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TG), X-ray diffraction (XRD) and N sorption analysis. Results showed that the as-synthesized MI-LPSPs exhibited a spherical morphology, favorable stability and large pore structure. The kinetic adsorption experiments showed that the MI-LPSPs could reach equilibrium within one hour and were described well by the pseudo second-order model, indicating that chemical adsorption might be the rate-limiting step. Meanwhile, the MI-LPSPs had a large binding capacity up to 162.82 mg g and high selectivity for the recognition of BSA. Moreover, such a high binding capacity and selectivity was retained after six runs, indicating a good stability and reusability of MI-LPSPs. Thus, it is expected that a simple synthetic methodology in the present study provides a promising pathway to prepare novel imprinted materials for efficient purification and separation of target proteins.
天然抗体广泛应用于生物医学分析、蛋白质分离和靶向药物递送等各种领域，但它们存在成本高和稳定性低的问题。在本研究中，我们开发了一种简便的方法，用于在多孔二氧化硅固体中构建类抗体结合位点，以基于大孔二氧化硅颗粒（LPSP）高效分离牛血清白蛋白（BSA）。这是通过接枝两种有机硅烷单体，3-氨丙基三乙氧基硅烷（APTES）和辛基三甲氧基硅烷（OTMS）来实现的，通过分子印迹技术与BSA提供氢键或疏水相互作用。通过扫描电子显微镜（SEM）、傅里叶变换红外（FT-IR）光谱、X射线光电子能谱（XPS）、热重分析（TG）、X射线衍射（XRD）和N吸附分析对所得的分子印迹大孔二氧化硅颗粒（MI-LPSP）进行了表征。结果表明，合成的MI-LPSP呈现球形形态、良好的稳定性和大孔结构。动力学吸附实验表明，MI-LPSP可在一小时内达到平衡，并用伪二级模型很好地描述，表明化学吸附可能是限速步骤。同时，MI-LPSP对BSA的识别具有高达162.82 mg g的大结合容量和高选择性。此外，在六次运行后仍保留了如此高的结合容量和选择性，表明MI-LPSP具有良好的稳定性和可重复使用性。因此，预计本研究中的简单合成方法为制备用于高效纯化和分离目标蛋白质的新型印迹材料提供了一条有前景的途径。
