Sun Jiachen, Zhang Yanxia, Li Bin, Gu Yong, Chen Liang
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P. R. China.
J Mater Chem B. 2017 Nov 28;5(44):8770-8779. doi: 10.1039/c7tb02043k. Epub 2017 Oct 30.
Stimulating the adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is crucial for the scaffold materials used in bone tissue engineering. In this study, a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was prepared, embedded with SF microspheres containing bone morphogenetic protein-2 (BMP-2) and surface-modified through chemical conjugation with the type I collagen-mimetic peptide GFOGER. Surface-bound GFOGER and sustained BMP-2 release promoted the adhesion and osteogenic differentiation, respectively, of BMSCs. Our results indicated that this system released low doses of BMP-2 in a sustained manner, thereby avoiding cost and safety problems. The biological responses to this scaffold were investigated both in vitro and in vivo. Our results indicated that the synergistic effect of GFOGER and BMP-2 promoted the adhesion and osteogenic differentiation of BMSCs, thus leading to complete bone bridging of rat cranial defects after 12 weeks of implantation. This system might provide a powerful platform for treating bone defects and for bone tissue engineering.
刺激骨髓间充质干细胞（BMSCs）的黏附和成骨分化对于骨组织工程中使用的支架材料至关重要。在本研究中，制备了丝素蛋白（SF）/纳米羟基磷灰石（nHAp）支架，其嵌入含有骨形态发生蛋白-2（BMP-2）的SF微球，并通过与I型胶原模拟肽GFOGER进行化学偶联进行表面修饰。表面结合的GFOGER和BMP-2的持续释放分别促进了BMSCs的黏附和成骨分化。我们的结果表明，该系统以持续的方式释放低剂量的BMP-2，从而避免了成本和安全问题。对该支架的生物学反应进行了体外和体内研究。我们的结果表明，GFOGER和BMP-2的协同作用促进了BMSCs的黏附和成骨分化，从而在植入12周后导致大鼠颅骨缺损完全骨桥接。该系统可能为治疗骨缺损和骨组织工程提供一个强大的平台。
