Cheng Xiamin, Zhang Ruoyu, Cai Xiaolei, Liu Bin
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore.
J Mater Chem B. 2017 May 21;5(19):3565-3571. doi: 10.1039/c7tb00436b. Epub 2017 Apr 26.
We report a fluorogenic probe for naked-eye sensing of hydrazine in solution and in the gaseous phase. The probe based on tetraphenylethylene (TPE) with aggregation-induced emission (AIE) characteristics shows OFF-ON fluorescence as observed by thin-layer chromatography (TLC) upon treatment with hydrazine. Specifically, the fluorescence of the probe was quenched due to the attached N[double bond, length as m-dash]N group, which can be reduced to -NH-NH- in the presence of hydrazine to turn on the fluorescence. The reduced intermediate can be easily oxidized in air to regenerate the original probe for recyclable usage. Both fluorometric and colorimetric readings were achieved by TLC with high sensitivity and excellent selectivity. This study thus represents a simple example of a reusable and naked-eye molecular probe for monitoring environmental hazards. Finally, the probe has also been applied to detect hydrazine in live cells.
我们报道了一种用于裸眼检测溶液和气相中肼的荧光探针。基于具有聚集诱导发光（AIE）特性的四苯乙烯（TPE）的探针，在用肼处理后通过薄层色谱（TLC）观察到呈现从关闭到开启的荧光。具体而言，由于连接的N=N基团，探针的荧光被淬灭，在肼存在下该基团可还原为-NH-NH-从而开启荧光。还原后的中间体在空气中容易被氧化以再生原始探针以便循环使用。通过TLC实现了荧光和比色读数，具有高灵敏度和出色的选择性。因此，这项研究代表了一个用于监测环境危害的可重复使用的裸眼分子探针的简单示例。最后，该探针还被应用于活细胞中肼的检测。
