Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Apr 15;195:136-141. doi: 10.1016/j.saa.2018.01.033. Epub 2018 Jan 12.
A rhodol-based fluorescent probe has been developed as a selective hydrazine chemosensor using levulinate as a recognition site. The rhodol levulinate probe (RL) demonstrated high selectivity and sensitivity toward hydrazine among other molecules. The chromogenic response of RL solution to hydrazine from colorless to pink could be readily observed by the naked eye, while strong fluorescence emission could be monitored upon excitation at 525 nm. The detection process occurred via a ring-opening process of the spirolactone initiated by hydrazinolysis, triggering the fluorescence emission with a 53-fold enhancement. The probe rapidly reacted with hydrazine in aqueous medium with the detection limit of 26 nM (0.83 ppb), lower than the threshold limit value (TLV) of 10 ppb suggested by the U.S. Environmental Protection Agency. Furthermore, RL-impregnated paper strips could detect hydrazine vapor. For biological applicability of RL, its membrane-permeable property led to bioimaging of hydrazine in live HepG2 cells by confocal fluorescence microscopy.
开发了一种基于香豆素的荧光探针，将戊酸酯作为识别位点，用作对肼的选择性化学传感器。香豆素戊酸酯探针（RL）在其他分子中对肼表现出高选择性和灵敏度。RL 溶液对肼的显色反应可以通过肉眼从无色变为粉红色来轻松观察到，而在 525nm 激发时可以监测到强荧光发射。检测过程是通过肼解引发螺内酯的开环反应发生的，从而使荧光发射增强了 53 倍。探针在水介质中与肼快速反应，检测限为 26nM（0.83ppb），低于美国环境保护署建议的 10ppb 阈值限值（TLV）。此外，RL 浸渍的纸条可以检测肼蒸气。RL 的膜通透性使其可以通过共聚焦荧光显微镜对活 HepG2 细胞中的肼进行生物成像，从而实现其在生物方面的应用。
