Wang Yulan, Hu Xiaoxia, Dai Jing, Wang Jie, Tan Yaning, Yang Xiangdong, Yang Shuang, Yuan Quan, Zhang Yufeng
State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, China.
J Mater Chem B. 2017 Sep 7;5(33):6794-6800. doi: 10.1039/c7tb01515a. Epub 2017 Aug 3.
Development of a cell-free scaffold with excellent mechanical properties and osteoconductivity is of significant need for bone regeneration. Herein, a reduced graphene oxide (rGO) functionalized hierarchical macro-mesoporous bioactive glass scaffold integrated with an osteoblast-specific aptamer is rationally designed to recruit and induce the rapid differentiation of osteoblasts for bone regeneration. This scaffold exhibits a macroporous structure with fully interconnected open pores and shows excellent mechanical properties with a Young's modulus of ∼80 kPa, which provides a strong scaffold to support the growth of osteoblasts and bone tissue regeneration. Furthermore, the scaffold displays good performance in accelerating osteoblast differentiation and promoting new bone formation. The osteoblast recruitment is achieved since the osteoblast-specific aptamer can specifically target osteoblasts with strong binding affinity. Micro-computed tomography and histological tests confirmed that the large bone defects fully heal with new plate-like-pattern bone appearing both peripherally and centrally, suggesting the outstanding bone regeneration performance of this cell-free and graphene functionalized scaffold. Considering the promising bioapplications of the graphene functionalized bioactive glass scaffold with osteoblast recruitment capacity, our strategy paves a way for the design of new bioactive functional materials for tissue regeneration and shows attractive prospects in targeted therapy.
开发具有优异机械性能和骨传导性的无细胞支架对于骨再生具有重大需求。在此，合理设计了一种与成骨细胞特异性适配体整合的还原氧化石墨烯（rGO）功能化分级大孔-介孔生物活性玻璃支架，以募集并诱导成骨细胞快速分化以实现骨再生。该支架呈现出具有完全相互连通的开放孔的大孔结构，并表现出优异的机械性能，杨氏模量约为80 kPa，为成骨细胞的生长和骨组织再生提供了强大的支架支撑。此外，该支架在加速成骨细胞分化和促进新骨形成方面表现良好。由于成骨细胞特异性适配体能够以强结合亲和力特异性靶向成骨细胞，从而实现了成骨细胞的募集。微型计算机断层扫描和组织学测试证实，大的骨缺损完全愈合，外周和中央均出现新的板层状骨，表明这种无细胞且石墨烯功能化的支架具有出色的骨再生性能。考虑到具有成骨细胞募集能力的石墨烯功能化生物活性玻璃支架具有广阔的生物应用前景，我们的策略为设计用于组织再生的新型生物活性功能材料铺平了道路，并在靶向治疗方面展现出诱人的前景。
