Cao Xiaowei, Shan Yibo, Tan Lianqiao, Yu Xi, Bao Min, Li Wei, Shi Hongcan
Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China.
J Mater Chem B. 2017 Aug 14;5(30):5983-5995. doi: 10.1039/c7tb01212h. Epub 2017 Jul 17.
Bone marrow mesenchymal stem cells (BMSCs) are multipotent stem cells, which play an important role in the repair of bone injury, angiogenesis, immune diseases, cancer invasion and metastasis. Therefore, increasing attention has been focused on the study of BMSCs. However, the identification and discrimination of the undifferentiated and differentiated BMSCs, which are closely related and morphologically similar, are still a challenge using traditional methods. In this study, we report a novel surface-enhanced Raman scattering (SERS) substrate based on hollow gold nanoflower (HAuNF)-decorated silicon wafers for distinguishing the differentiation of BMSCs. The flower-like, hollow Au nanoparticles were successfully synthesized by a seed-mediated growth approach. We demonstrated that fabricated HAuNF substrates had very good reproducibility, homogeneous SERS activity and a high SERS effect. Using HAuNF substrates as a high-performance in vitro sensing platform allowed us to monitor the changes in the cellular biochemical composition during the differentiation of BMSCs. SERS spectra were analyzed using principal component analysis (PCA), which successfully segregated the subtypes of BMSCs. Furthermore, the results of biological assay suggested that the adipogenic inductor and the osteogenic inductor could induce the differentiation of BMSCs into adipocytes and osteocytes, respectively. The SERS technique based on HAuNF substrates provides a sensitive, efficient and non-invasive detection method for studying the differentiation of stem cells.
骨髓间充质干细胞（BMSCs）是多能干细胞，在骨损伤修复、血管生成、免疫疾病、癌症侵袭和转移中发挥着重要作用。因此，对BMSCs的研究越来越受到关注。然而，使用传统方法来鉴定和区分未分化和已分化的BMSCs仍然具有挑战性，因为它们密切相关且形态相似。在本研究中，我们报道了一种基于中空金纳米花（HAuNF）修饰硅片的新型表面增强拉曼散射（SERS）基底，用于区分BMSCs的分化。通过种子介导生长法成功合成了花状中空金纳米颗粒。我们证明所制备的HAuNF基底具有非常好的重现性、均匀的SERS活性和高SERS效应。使用HAuNF基底作为高性能的体外传感平台，使我们能够监测BMSCs分化过程中细胞生化成分的变化。利用主成分分析（PCA）对SERS光谱进行分析，成功地分离了BMSCs的亚型。此外，生物学检测结果表明，成脂诱导剂和成骨诱导剂可分别诱导BMSCs分化为脂肪细胞和骨细胞。基于HAuNF基底的SERS技术为研究干细胞分化提供了一种灵敏、高效且无创的检测方法。
