Xu Jiating, Lv Ruichan, Du Shaokang, Gai Shili, He Fei, Yang Dan, Yang Piaoping
Key Laboratory of Superlight Materials and Surface Technology, Ministry of Education, College of Material Sciences and Chemical Engineering, Harbin Engineering University, Harbin, 150001, P. R. China.
J Mater Chem B. 2016 Jun 21;4(23):4138-4146. doi: 10.1039/c6tb00714g. Epub 2016 May 23.
To enhance the total emission intensity, particularly the red emission of Yb,Er co-doped nanoparticles for red light activated photodynamic therapy (PDT), we doped Mn ions into the NaGdF:Yb,Er core, and subsequently coated the NaGdF:Yb active shell to fabricate core-shell structured, up-conversion nanoparticles of NaGdF:Yb,Er,Mn@NaGdF:Yb (abbreviated as UCNPs). A novel and facile encapsulation method with gelatin has been proposed to transfer oleic acid (OA) stabilized UCNPs into an aqueous solution and simultaneously decorate zinc phthalocyanine (ZnPc) photosensitizer molecules. In the encapsulation process, ZnPc molecules are wrapped in the interlaced net structure of the peptide chain from gelatin, forming the UCNPs@gel-ZnPc nanocomposite. The nanoplatform has high emission intensity and excellent biocompatibility, as was expected. More importantly, the enhanced red emission of UCNPs has significant overlap with the UV absorbance of ZnPc; therefore, it can effectively activate the sensitizer to produce a large amount of singlet oxygen reactive oxygen species (ROS, O) to kill cancer cells, which has evidently been verified by the in vitro results. Combined with the inherent up-conversion luminescence (UCL) imaging properties, this UCNPs@gel-ZnPc nanoplatform could have potential application in PDT and imaging fields.
为了提高用于红光激活光动力疗法（PDT）的Yb、Er共掺杂纳米粒子的总发射强度，特别是Yb的红色发射，我们将Mn离子掺杂到NaGdF:Yb、Er核中，随后包覆NaGdF:Yb活性壳层，以制备核壳结构的NaGdF:Yb、Er、Mn@NaGdF:Yb上转换纳米粒子（简称为UCNPs）。提出了一种新颖且简便的明胶包封方法，将油酸（OA）稳定的UCNPs转移到水溶液中，同时修饰锌酞菁（ZnPc）光敏剂分子。在包封过程中，ZnPc分子被包裹在明胶肽链的交错网状结构中，形成UCNPs@gel-ZnPc纳米复合材料。正如预期的那样，该纳米平台具有高发射强度和优异的生物相容性。更重要的是，UCNPs增强的红色发射与ZnPc的紫外吸收有显著重叠；因此，它可以有效地激活敏化剂产生大量单线态氧活性氧物种（ROS，O）来杀死癌细胞，体外实验结果已对此进行了明显验证。结合其固有的上转换发光（UCL）成像特性，这种UCNPs@gel-ZnPc纳米平台在PDT和成像领域可能具有潜在应用。
