ARC Research Hub for Australian Steel Manufacturing, Wollongong, NSW 2522, Australia.
Intelligent Polymer Research Institute, ARC Centre of Excellence for Electromaterials Science, AIIM Facility, Innovation Campus, University of Wollongong, Wollongong, NSW 2500, Australia.
Biointerphases. 2020 Apr 7;15(2):021009. doi: 10.1116/1.5143141.
This work examines the antifouling effect of quaternary ammonium silane (QAS) grafted from coatings of silica nanoparticles (SiNPs), independently and in combination with a zwitterionic sulfobetaine (SB) silane. The binding of QAS to the SiNP coatings was monitored using quartz crystal microgravimetry with dissipation monitoring (QCM-D) under varied pH and solution concentrations. Adsorption of bovine serum albumin protein was reduced on QAS modified SiNP coatings prepared under alkaline conditions due to the proposed generation of a pseudozwitterionic interface, where the underlying SiNP surface presents an anionic charge at high pH. Significant reductions in protein binding were achieved at low functionalization concentrations and short modification times. Additionally, SiNP coatings modified with a combination of QAS and SB chemistries were investigated. Surface modifications were performed sequentially, varying silane concentration and order of addition, and monitored using QCM-D. Dual-functionalized surfaces presented enhanced resistance to protein adsorption compared to QAS or SB modified surfaces alone, even at low functionalization concentrations. The antiadhesive and antibacterial properties of functionalized surfaces were investigated by challenging the surfaces against the bacterium Escherichia coli. All dual-functionalized coatings showed equal or reduced bacterial adhesion compared to QAS and SB functionalizations alone, while coatings functionalized with high concentrations of combined chemistries reduced the adhesion of bacteria by up to 95% compared to control SiNP surfaces.
这项工作研究了季铵硅烷（QAS）从二氧化硅纳米粒子（SiNPs）涂层接枝的防污效果，独立地和与两性离子磺酸甜菜碱（SB）硅烷结合使用。使用石英晶体微天平（QCM-D）在不同 pH 值和溶液浓度下监测 QAS 与 SiNP 涂层的结合情况。在碱性条件下，由于拟生成假两性离子界面，底层 SiNP 表面在高 pH 值下带负电荷，因此 QAS 改性的 SiNP 涂层上的牛血清白蛋白（BSA）蛋白吸附减少。在低官能化浓度和短修饰时间下，实现了显著降低蛋白质结合。此外，还研究了用 QAS 和 SB 化学组合修饰的 SiNP 涂层。表面修饰顺序进行，改变硅烷浓度和添加顺序，并使用 QCM-D 进行监测。与单独的 QAS 或 SB 改性表面相比，双官能化表面表现出增强的抗蛋白质吸附能力，即使在低官能化浓度下也是如此。通过用细菌大肠杆菌挑战表面，研究了功能化表面的抗粘附和抗菌性能。与单独的 QAS 和 SB 官能化相比，所有双官能化涂层显示出相等或降低的细菌粘附，而用高浓度组合化学修饰的涂层使细菌的粘附减少了 95%，与对照 SiNP 表面相比。
