Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.
Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Sci Rep. 2020 Apr 7;10(1):5996. doi: 10.1038/s41598-020-62876-1.
The identification of host cell factors for virus entry is useful for the molecular explanation of viral tropisms and often leads to a more profound understanding of virus-induced diseases. Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS virus. No countermeasures against the disease exist. In this report, we show an efficient method using virus-like particles for the functional screening of a cellular cDNA library to identify SFTS virus entry factors. Two variants encoding dendritic cell-specific ICAM-3 grabbing non-integrin related (DC-SIGNR), a calcium-dependent lectin known to enhance SFTS virus infection, were successfully identified from a human liver cDNA library. We will discuss applications for yet unidentified factor(s) for SFTS virus entry and for entry factor(s) for other viruses related to SFTS virus.
宿主细胞因子的鉴定对于病毒的嗜性的分子解释很有用，并且常常会深入了解病毒引起的疾病。发热伴血小板减少综合征（SFTS）是一种由 SFTS 病毒引起的新发传染病。目前尚无针对该疾病的对策。在本报告中，我们展示了一种使用病毒样颗粒进行细胞 cDNA 文库功能筛选以鉴定 SFTS 病毒进入因子的有效方法。从人肝 cDNA 文库中成功鉴定出两种编码树突状细胞特异性 ICAM-3 抓取非整合素相关（DC-SIGNR）的变体，该糖蛋白已知可增强 SFTS 病毒的感染。我们将讨论 SFTS 病毒进入相关的未知因子（s）以及与 SFTS 病毒相关的其他病毒进入因子（s）的应用。
