Taniguchi Satoshi, Fukuma Aiko, Tani Hideki, Fukushi Shuetsu, Saijo Masayuki, Shimojima Masayuki
Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Special Pathogens Laboratory, Department of Virology I, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
J Virol Methods. 2017 Jun;244:4-10. doi: 10.1016/j.jviromet.2017.01.005. Epub 2017 Jan 9.
Severe fever with thrombocytopenia syndrome (SFTS) is a recently-discovered, potentially fatal infectious disease caused by SFTS virus (SFTSV). Due to the inability of SFTSV to make clear cytopathic effects (CPE) in cell culture, titration and neutralization assays of the virus require immunostaining of inoculated cells; consequently, the assays are time-consuming and expensive. In this report, we demonstrate the use of a highly-passaged SFTSV strain, p50-2, in a neutralization assay, which made clear plaques in inoculated Vero cells under neutral red staining. Furthermore, we performed molecular analyses to determine the characteristics of the strain. The results suggested that a single amino acid mutation within the viral glycoprotein conferred the ability to make clear plaques to SFTSV.
发热伴血小板减少综合征（SFTS）是一种最近发现的、由SFTS病毒（SFTSV）引起的潜在致命性传染病。由于SFTSV在细胞培养中无法产生明显的细胞病变效应（CPE），病毒的滴定和中和试验需要对接种细胞进行免疫染色；因此，这些试验既耗时又昂贵。在本报告中，我们展示了在中和试验中使用高度传代的SFTSV毒株p50-2，该毒株在中性红染色下于接种的Vero细胞中形成了清晰的蚀斑。此外，我们进行了分子分析以确定该毒株的特征。结果表明，病毒糖蛋白内的单个氨基酸突变赋予了SFTSV产生清晰蚀斑的能力。
