Grupo de Ciencias Básicas, Doctorado en Ciencias de la Salud, Escuela de Graduados, Universidad CES, Medellín, Colombia.
Línea de Investigación en Lepra, Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia.
Front Immunol. 2020 Mar 19;11:368. doi: 10.3389/fimmu.2020.00368. eCollection 2020.
Leprosy is an infectious disease caused by , a debilitating disease that affects the skin and peripheral nerves. It is possible that tissue changes during infection with leprosy are related to alterations in the activity of the Notch signaling pathway, an innate signaling pathway in the physiology of the skin and peripheral nerves. This is a descriptive observational study. Thirty skin biopsies from leprosy patients and 15 from individuals with no history of this disease were evaluated. In these samples, gene expressions of cellular components associated with the Notch signaling pathway, , and , were evaluated using q-PCR, and protein expression was evaluated using immunohistochemistry of Runx-1 and Hes-1. Changes were observed in the transcription of Notch signaling pathway components; Hes-1 was downregulated and Runx-1 upregulated in the skin of infected patients. These results were confirmed by immunohistochemistry, where reduction of Hes-1 expression was found in the epidermis, eccrine glands, and hair follicles. Increased expression of Runx-1 was found in inflammatory cells in the dermis of infected patients; however, it is not related to tissue changes. With these results, a multivariate analysis was performed to determine the causes of transcription factor Hes-1 reduction. It was concluded that tissue inflammation was the main cause. The tissue changes found in the skin of infected patients could be associated with a reduction in the expression of Hes-1, a situation that would promote the survival and proliferation of in this tissue.
麻风病是一种由 引起的传染病，是一种使人虚弱的疾病，会影响皮肤和外周神经。麻风病感染过程中的组织变化可能与 Notch 信号通路活性的改变有关，Notch 信号通路是皮肤和外周神经生理学中的一种固有信号通路。 这是一项描述性观察研究。评估了 30 例麻风病患者和 15 例无此病病史的个体的皮肤活检。在这些样本中，使用 q-PCR 评估了与 Notch 信号通路相关的细胞成分的基因表达 ， ，和 ，并用免疫组织化学评估了 Runx-1 和 Hes-1 的蛋白表达。 Notch 信号通路成分的转录发生了变化；感染患者的皮肤中 Hes-1 下调，Runx-1 上调。免疫组织化学证实了这一点，在表皮、外分泌腺和毛囊中发现 Hes-1 表达减少。在感染患者的真皮炎症细胞中发现 Runx-1 表达增加；然而，这与组织变化无关。根据这些结果，进行了多变量分析以确定转录因子 Hes-1 减少的原因。结论是组织炎症是主要原因。感染患者皮肤中的组织变化可能与 Hes-1 表达减少有关，这种情况会促进 在该组织中的存活和增殖。
