Departments of Physiology and Histology and Embryology, School of Medicine, Marmara University, İstanbul, Turkey.
Histology and Embryology, School of Medicine, Marmara University, İstanbul, Turkey.
J Physiol. 2020 Jun;598(12):2355-2370. doi: 10.1113/JP279577. Epub 2020 May 18.
A moderate level of exercise has beneficial effects for the prevention of gastric ulcers. Although regular aerobic exercise was shown to elevate serum oxytocin levels and exogenously administered oxytocin exerts an anti-ulcer activity, the role of endogenous oxytocin in the gastroprotective effects of exercise has not yet been elucidated. We showed that increased anxiety and oxidative gastric damage induced by gastric ulcers were reversed in pre-exercised rats, while reduced hypothalamic oxytocin expression and decreased myenteric oxytocin receptor expression due to gastric ulcers were abolished by exercise. We also reported that the blockade of oxytocin receptors exaggerated gastric damage in exercised rats with ulcers. Our data establish that endogenous oxytocin is the key mediator in the beneficial effects of regular physical activity in alleviating gastric injury.
Exercise increases serum oxytocin levels and exogenous oxytocin exerts an anti-ulcer activity; but the role of oxytocin in the protective effects of exercise against gastric ulcers has not yet been evaluated. This study was designed to investigate the impact of regular swimming exercise on oxidative gastric injury, and the role of oxytocin receptor activity in the anxiolytic and anti-inflammatory actions of exercise. Adult Wistar albino rats of both sexes performed swimming exercise (30 min/day, 5 days) or stayed sedentary. At the end of the 6-week exercise/sedentary protocol, rats were injected intraperitoneally with atosiban (0.1 mg/kg/day) or saline for 4 days. On the 5th day, under anaesthesia, acetic acid (ulcer) or saline (sham) was applied onto the gastric serosa and the treatments were continued. On the 9th day, anxiety levels were determined; gastric blood flow was measured, and blood, gastric and brain tissues were obtained. Induction of ulcers in sedentary rats increased anxiety and serum corticosterone levels; but reduced gastric blood flow and resulted in apoptosis and oxidative gastric damage with increased cytokine expressions. However, when ulcers were induced in pre-exercised rats, behavioural and biochemical alterations due to gastric damage were reversed. The inhibition of oxytocin receptors by atosiban exaggerated pro-inflammatory cytokine expressions and gastric lipid peroxidation in the stomachs of exercised rats with ulcers. When rats had regularly exercised prior to ulcer induction, reductions in the immunolabelling of hypothalamic oxytocin and myenteric oxytocin receptors were abolished, suggesting that exercise-induced alleviation of gastric injury may involve the reversal of down-regulated oxytocinergic activity.
适度运动有益于预防胃溃疡。虽然有研究表明，有规律的有氧运动可提高血清催产素水平，外源性催产素具有抗溃疡活性，但运动对胃的保护作用中的内源性催产素的作用尚未阐明。我们发现，运动可逆转由胃溃疡引起的焦虑增加和氧化胃损伤，而由于胃溃疡导致的下丘脑催产素表达减少和肌间神经丛催产素受体表达减少则可被运动消除。我们还报告称，在有溃疡的运动大鼠中，阻断催产素受体可加重胃损伤。我们的数据表明，内源性催产素是规律体育活动缓解胃损伤的有益作用的关键介质。
运动可增加血清催产素水平，外源性催产素具有抗溃疡活性；但运动对胃的保护作用中的内源性催产素的作用尚未得到评估。本研究旨在研究有规律的游泳运动对氧化胃损伤的影响，以及催产素受体活性在运动的抗焦虑和抗炎作用中的作用。成年雄性和雌性 Wistar 白化大鼠进行游泳运动（30 分钟/天，5 天）或保持久坐不动。在 6 周的运动/久坐不动方案结束时，大鼠每天腹膜内注射阿托西班（0.1mg/kg/天）或生理盐水 4 天。在第 5 天，在麻醉下，将乙酸（溃疡）或生理盐水（假手术）应用于胃浆膜，并且继续进行治疗。在第 9 天，测定焦虑水平；测量胃血流，并获得血液、胃和脑组织。在久坐不动的大鼠中诱导溃疡会增加焦虑和血清皮质酮水平；但降低胃血流，并导致凋亡和氧化胃损伤，同时细胞因子表达增加。然而，当在预先运动的大鼠中诱导溃疡时，由于胃损伤导致的行为和生化改变则得到逆转。阿托西班抑制催产素受体可在运动大鼠的溃疡胃中放大促炎细胞因子的表达和胃脂质过氧化。当大鼠在诱导溃疡之前有规律地运动时，下丘脑催产素和肌间神经丛催产素受体的免疫标记减少被消除，这表明运动诱导的胃损伤缓解可能涉及逆转下调的催产素能活性。
