Guragac Dereli F Tugce, Genc Yasin, Saracoglu Iclal, Kupeli Akkol Esra
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330, Etiler, Ankara, Turkey.
Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, 06100, Sihhiye, Ankara, Turkey.
Z Naturforsch C J Biosci. 2020 Mar 26;75(3-4):121-128. doi: 10.1515/znc-2020-0008.
Plants of the Plantago genus are widely used in Turkish folk medicine especially for the treatment of wound, abscess, and inflammation. The aqueous extract and five phenylethanoid glycosides acteoside (1), arenarioside (2), echinacoside (3), isoacteoside (4), and leucosceptoside A (5) isolated from the aerial parts and roots of Plantago holosteum Scop. (Plantaginaceae) were tested for their possible inhibitory activity against hyaluronidase, elastase, and collagenase, related to wound pathogenesis. Even though the aqueous extract prepared from the aerial parts (36.26%) and roots (47.01%) and the isolated compounds acteoside (29.13%), echinacoside (28.73%), and isoacteoside (31.69%) exerted a notable inhibition, arenarioside and leucosceptoside A were found inactive in the hyaluronidase enzyme inhibition test. Similar results were obtained from the collagenase enzyme inhibition test. The aqueous extract prepared from the aerial parts (31.09%) and roots (35.17%), echinacoside (25.13%), and isoacteoside (23.85%) exerted a notable inhibition in this test. However, none of the extracts and isolated compounds displayed elastase enzyme inhibitory activity. The experimental data demonstrated that P. holosteum displayed a remarkable enzyme inhibitory activity against hyaluronidase and collagenase. This paper is the first report regarding the in vitro enzyme inhibitory activity of P. holosteum.
车前属植物在土耳其民间医学中广泛应用，尤其用于治疗伤口、脓肿和炎症。从车前（Plantaginaceae）地上部分和根部分离得到的水提取物以及五种苯乙醇苷类化合物，即毛蕊花糖苷（1）、紫斑风铃草苷（2）、松果菊苷（3）、异毛蕊花糖苷（4）和白花败酱苷A（5），针对与伤口发病机制相关的透明质酸酶、弹性蛋白酶和胶原酶的潜在抑制活性进行了测试。尽管从地上部分（36.26%）和根部（47.01%）制备的水提取物以及分离得到的化合物毛蕊花糖苷（29.13%）、松果菊苷（28.73%）和异毛蕊花糖苷（31.69%）表现出显著抑制作用，但在透明质酸酶抑制试验中发现紫斑风铃草苷和白花败酱苷A无活性。胶原酶抑制试验也得到了类似结果。在该试验中，从地上部分（31.09%）和根部（35.17%）制备的水提取物、松果菊苷（25.13%）和异毛蕊花糖苷（23.85%）表现出显著抑制作用。然而，所有提取物和分离得到的化合物均未表现出弹性蛋白酶抑制活性。实验数据表明，车前对透明质酸酶和胶原酶具有显著的酶抑制活性。本文是关于车前体外酶抑制活性的首次报道。
