Karatoprak Gökçe Şeker, Göger Fatih, Çelik İsmail, Budak Ümit, Akkol Esra Küpeli, Aschner Michael
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey.
S Afr J Bot. 2022 May;146:36-47. doi: 10.1016/j.sajb.2021.09.033. Epub 2021 Oct 9.
Celep & Doğan is an endemic species of Turkey. To our knowledge, the number of studies on biological activities and phytochemical profiling of this plant is quite limited. Therefore, this study aimed to analyze its activities and phytochemical content in detail. The qualitative-quantitative compositions were determined via spectrophotometric and chromatographic (LC-MS/MS and HPLC) techniques. 1,1-Diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2'-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging and ascorbate-iron (III)-catalyzed phospholipid peroxidation experiments were performed to measure antioxidant capacity. Hyaluronidase, collagenase, and elastase enzyme inhibition tests were determined using a spectrophotometer. Antiproliferative activity was evaluated in human lung cancer (A549) and human breast cancer (MCF7) cells. The murine fibroblast (L929) cell line was used as a normal control cell. While the subextract rich in phenolic compounds was -butanol extract, rosmarinic acid was defined as the main secondary metabolite. The highest antioxidant activity observed for the -butanol subextract included the following: DPPH EC50: 0.08±0.00 mg/mL, TEAC/ABTS: 2.19±0.09 mmol/L Trolox, MDA EC50: 0.42±0.03 mg/mL. The methanolic extract, the ethyl acetate, and -butanol subextracts displayed significant inhibitory activity on collagenase, while the other subextracts did not show any inhibitory activity on hyaluronidase and elastase. Due to strong interactions with their active sites, molecular docking showed luteolin 7-glucuronide, apigenin 7-glucuronide, and luteolin 5-glucoside had the highest binding affinity with target enzymes. The chloroform subextract showed significant cytotoxicity in all cell lines. These novel results revealed that has strong antioxidant, collagenase enzyme inhibitory, and cytotoxic potential.
塞莱普&多安是土耳其的特有物种。据我们所知，关于这种植物生物活性和植物化学特征分析的研究数量相当有限。因此，本研究旨在详细分析其活性和植物化学成分。通过分光光度法和色谱法（液相色谱-串联质谱法和高效液相色谱法）测定其定性和定量组成。进行1,1-二苯基-2-苦基肼自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）自由基清除以及抗坏血酸-铁（III）催化的磷脂过氧化实验以测定抗氧化能力。使用分光光度计测定透明质酸酶、胶原酶和弹性蛋白酶的酶抑制试验。在人肺癌（A549）和人乳腺癌（MCF7）细胞中评估抗增殖活性。小鼠成纤维细胞（L929）细胞系用作正常对照细胞。富含酚类化合物的亚提取物是正丁醇提取物，迷迭香酸被确定为主要次生代谢产物。正丁醇亚提取物观察到的最高抗氧化活性如下：DPPH半数有效浓度（EC50）：0.08±0.00毫克/毫升，TEAC/ABTS：2.19±0.09毫摩尔/升 Trolox，丙二醛（MDA）EC50：0.42±0.03毫克/毫升。甲醇提取物、乙酸乙酯和正丁醇亚提取物对胶原酶显示出显著的抑制活性，而其他亚提取物对透明质酸酶和弹性蛋白酶未显示任何抑制活性。由于与它们的活性位点有强烈相互作用，分子对接显示木犀草素7-葡萄糖醛酸苷、芹菜素7-葡萄糖醛酸苷和木犀草素5-葡萄糖苷与靶酶具有最高的结合亲和力。氯仿亚提取物在所有细胞系中均显示出显著的细胞毒性。这些新结果表明该植物具有强大的抗氧化、胶原酶抑制和细胞毒性潜力。
