Landfill leachate treatment through the combination of genetically engineered bacteria Rhodococcus erythropolis expressing Nirs and AMO and membrane filtration processes.

This study developed a process of genetically engineered bacteria Rhodococcus erythropolis expressing Nirs and AMO combined with membrane bioreactor (MBR), nanofiltration (NF) and reverse osmosis (RO) membrane (pRho-NA-MNR) for advanced treatment of landfill leachate. Results demonstrated that pRho-NA-MNR presented higher removal rate of chemical oxygen demand (COD), biological oxygen demand (BOD), ammonia nitrogen (N-NH), total nitrogen (TN) and total organic carbon (TOC) than activated sludge (AS-MNR) system. Administration of pRho-NA increased nitrification by converting N-NH to nitrite (N-NO) and Nitrate (N-NO), and promoting denitrification by converting N-NO to nitrogen (N) in the landfill leachate treatment, promoted the pH control, increased sludge activity and effluent yield, shortened phase length adaptation under alternating aerobic-anoxic conditions. pRho-NA increased the nitration and denitrifying rate in the aerobic and anaerobic stage in the system by increasing Cyt cd1 and Cyt c expression in the activated sludge. Nitrogen removal by nitrification and denitrification was positively correlated to the concentration of Nirs and AMO expression. Treatment with pRho-NA promoted pollutant removal efficiency of membrane bioreactor, nanofiltration and reverse osmosis membrane processes in landfill leachate. In conclusion, data suggest that pRho-NA-MNR facilitates the formation of granular sludge and enhances comparable removal of nitrogen and organic compounds, indicating the practice of this process should be considered in landfill leachate treatment system.