Ni Jing, Yang Zhuan, Zhang Yue, Ma Ying, Xiong Hejian, Jian Wenjie
Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen, China.
College of Ocean Food and Biological Engineering, Jimei University, Xiamen, China.
Front Microbiol. 2022 Dec 8;13:1048216. doi: 10.3389/fmicb.2022.1048216. eCollection 2022.
Silver nanoparticles (AgNPs) have the potential to be used in aquaculture, but their influence on the growth and health of aquatic organisms has not been extensively investigated. In this study, the abalone viscera hydrolysates decorated AgNPs (AVH-AgNPs) were dispersed into aquaculture water at different concentrations (0, 6, 9, and 18 μg/l) to evaluate the biological effects on zebrafish (Danio rerio). The results showed that the AVH-AgNPs treatments of 6 and 9 μg/l promoted the growth and did not cause obvious damage to the gills, intestines, and livers of zebrafish. All the treatments induced catalase (CAT) and superoxide dismutase (SOD) activities and increased glutathione (GSH) content in the livers and upregulated the expression of immune related genes. The effects of 9 and 18 μg/l AVH-AgNPs treatments were more obvious. After AVH-AgNPs treatment, the abundances of some potential pathogens, such as species Plesimonas shigelloides and Pseudomonas alcaligenes and genus Flavobacterium decreased significantly. In contrast, the abundance of some beneficial bacteria that can degrade pollutants and toxins (e.g., Rhodococcus erythropolis) increased significantly. Thus, the application of low concentrations (6 ~ 18 μg/l) of AVH-AgNPs in aquaculture water is relatively safe and has a positive effect on zebrafish farming.
银纳米颗粒（AgNPs）有潜力应用于水产养殖，但其对水生生物生长和健康的影响尚未得到广泛研究。在本研究中，将鲍鱼内脏水解物修饰的银纳米颗粒（AVH-AgNPs）以不同浓度（0、6、9和18μg/l）分散到水产养殖水中，以评估对斑马鱼（Danio rerio）的生物学效应。结果表明，6和9μg/l的AVH-AgNPs处理促进了斑马鱼的生长，且未对其鳃、肠道和肝脏造成明显损伤。所有处理均诱导了肝脏中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性，增加了谷胱甘肽（GSH）的含量，并上调了免疫相关基因的表达。9和18μg/l的AVH-AgNPs处理效果更明显。经AVH-AgNPs处理后，一些潜在病原体，如类志贺邻单胞菌、产碱假单胞菌和黄杆菌属的丰度显著降低。相反，一些能够降解污染物和毒素的有益细菌（如红平红球菌）的丰度显著增加。因此，在水产养殖水中应用低浓度（6~18μg/l）的AVH-AgNPs相对安全，对斑马鱼养殖有积极作用。
