用于提高诊断准确性的超敏艰难梭菌毒素检测
Ultrasensitive Clostridioides difficile Toxin Testing for Higher Diagnostic Accuracy.
作者信息
Sandlund Johanna, Davies Kerrie, Wilcox Mark H
机构信息
Fluxus, Inc., Santa Clara, California, USA.
Healthcare Associated Infections Research Group, Leeds Teaching Hospitals NHS Trust and University of Leeds, Leeds, United Kingdom.
出版信息
J Clin Microbiol. 2020 May 26;58(6). doi: 10.1128/JCM.01913-19.
Abstract
Currently available diagnostic tests for infection (CDI) lack specificity or sensitivity, which has led to guideline recommendations for multistep testing algorithms. Ultrasensitive assays for detection of toxins provide measurements of disease-specific markers at very low concentrations. These assays may show improved accuracy compared to that of current testing methods and offer a potential standalone solution for CDI diagnosis, although large studies of clinical performance and accuracy are lacking.
摘要
目前可用的艰难梭菌感染（CDI）诊断测试缺乏特异性或敏感性，这导致了对多步骤测试算法的指南建议。用于检测毒素的超灵敏测定法能够在非常低的浓度下测量疾病特异性标志物。与当前的测试方法相比，这些测定法可能显示出更高的准确性，并为CDI诊断提供了一种潜在的独立解决方案，尽管缺乏关于临床性能和准确性的大型研究。
相似文献
1
Ultrasensitive Clostridioides difficile Toxin Testing for Higher Diagnostic Accuracy.用于提高诊断准确性的超敏艰难梭菌毒素检测
J Clin Microbiol. 2020 May 26;58(6). doi: 10.1128/JCM.01913-19.
2
Evaluation of Cycle Threshold, Toxin Concentration, and Clinical Characteristics of Clostridioides difficile Infection in Patients with Discordant Diagnostic Test Results.诊断试验结果不一致的艰难梭菌感染患者的循环阈值、毒素浓度及临床特征评估
J Clin Microbiol. 2020 Apr 23;58(5). doi: 10.1128/JCM.01681-19.
3
High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing.高敏艰难梭菌毒素检测与应用谷氨酸脱氢酶和毒素酶联免疫吸附试验及细胞毒素检测的艰难梭菌实验室算法的高度一致性。
J Clin Microbiol. 2020 Jan 28;58(2). doi: 10.1128/JCM.01629-19.
4
Host Immune Markers Distinguish Clostridioides difficile Infection From Asymptomatic Carriage and Non-C. difficile Diarrhea.宿主免疫标志物可区分艰难梭菌感染与无症状携带和非艰难梭菌性腹泻。
Clin Infect Dis. 2020 Mar 3;70(6):1083-1093. doi: 10.1093/cid/ciz330.
5
Ultrasensitive Detection of Clostridioides difficile Toxins A and B by Use of Automated Single-Molecule Counting Technology.利用自动化单分子计数技术检测艰难梭菌毒素 A 和 B 的超敏性。
J Clin Microbiol. 2018 Oct 25;56(11). doi: 10.1128/JCM.00908-18. Print 2018 Nov.
6
Ultrasensitive Detection of Clostridioides difficile Toxins in Stool by Use of Single-Molecule Counting Technology: Comparison with Detection of Free Toxin by Cell Culture Cytotoxicity Neutralization Assay.利用单分子计数技术检测粪便中的艰难梭菌毒素：与细胞培养细胞毒性中和试验检测游离毒素的比较。
J Clin Microbiol. 2019 Oct 23;57(11). doi: 10.1128/JCM.00719-19. Print 2019 Nov.
7
Pediatric Clostridioides difficile Infection: Diagnosis and Diagnostic Stewardship.小儿艰难梭菌感染：诊断和诊断管理。
J Pediatric Infect Dis Soc. 2021 Nov 17;10(Supplement_3):S16-S21. doi: 10.1093/jpids/piab054.
8
Prospective Evaluation of the mariPOC Test for Detection of Clostridioides difficile Glutamate Dehydrogenase and Toxins A/B.用于检测艰难梭菌谷氨酸脱氢酶及毒素A/B的mariPOC检测的前瞻性评估
J Clin Microbiol. 2020 Mar 25;58(4). doi: 10.1128/JCM.01872-19.
9
Absence of Toxemia in Clostridioides difficile Infection: Results from Ultrasensitive Toxin Assay of Serum.艰难梭菌感染中无中毒症：血清超敏毒素检测的结果。
Dig Dis Sci. 2021 Oct;66(10):3303-3306. doi: 10.1007/s10620-020-06683-8. Epub 2020 Nov 8.
10
Ultrasensitive Detection of Clostridium difficile Toxins Reveals Suboptimal Accuracy of Toxin Gene Cycle Thresholds for Toxin Predictions.超敏检测艰难梭菌毒素揭示毒素基因循环阈值对毒素预测的准确性欠佳。
J Clin Microbiol. 2019 May 24;57(6). doi: 10.1128/JCM.01885-18. Print 2019 Jun.
引用本文的文献
1
infection: an update.感染：最新进展。
Infez Med. 2024 Sep 1;32(3):280-291. doi: 10.53854/liim-3203-3. eCollection 2024.
2
infection: history, epidemiology, risk factors, prevention, clinical manifestations, treatment, and future options.感染：历史、流行病学、风险因素、预防、临床表现、治疗和未来选择。
Clin Microbiol Rev. 2024 Jun 13;37(2):e0013523. doi: 10.1128/cmr.00135-23. Epub 2024 Feb 29.
3
Clostridioides (Clostridium) difficile: A silent nosocomial pathogen.艰难梭菌（梭状芽孢杆菌）：一种沉默的医院病原体。
Saudi Med J. 2023 Sep;44(9):825-835. doi: 10.15537/smj.2023.44.9.20230216.
4
Characterization of the gut microbiome of patients with infection, patients with non- diarrhea, and -colonized patients. 感染患者、非腹泻患者和定植患者的肠道微生物组特征。
Front Cell Infect Microbiol. 2023 Apr 12;13:1130701. doi: 10.3389/fcimb.2023.1130701. eCollection 2023.
本文引用的文献
1
High Agreement Between an Ultrasensitive Clostridioides difficile Toxin Assay and a C. difficile Laboratory Algorithm Utilizing GDH-and-Toxin Enzyme Immunoassays and Cytotoxin Testing.高敏艰难梭菌毒素检测与应用谷氨酸脱氢酶和毒素酶联免疫吸附试验及细胞毒素检测的艰难梭菌实验室算法的高度一致性。
J Clin Microbiol. 2020 Jan 28;58(2). doi: 10.1128/JCM.01629-19.
2
Increased Clinical Specificity with Ultrasensitive Detection of Clostridioides difficile Toxins: Reduction of Overdiagnosis Compared to Nucleic Acid Amplification Tests.超敏检测艰难梭菌毒素可提高临床特异性：与核酸扩增检测相比，减少过度诊断。
J Clin Microbiol. 2019 Oct 23;57(11). doi: 10.1128/JCM.00945-19. Print 2019 Nov.
3
Ultrasensitive Detection of Clostridioides difficile Toxins in Stool by Use of Single-Molecule Counting Technology: Comparison with Detection of Free Toxin by Cell Culture Cytotoxicity Neutralization Assay.利用单分子计数技术检测粪便中的艰难梭菌毒素：与细胞培养细胞毒性中和试验检测游离毒素的比较。
J Clin Microbiol. 2019 Oct 23;57(11). doi: 10.1128/JCM.00719-19. Print 2019 Nov.
4
Toxin A-Predominant Pathogenic Clostridioides difficile: A Novel Clinical Phenotype.产毒为主的艰难梭菌：一种新的临床表型。
Clin Infect Dis. 2020 Jun 10;70(12):2628-2633. doi: 10.1093/cid/ciz727.
5
The perils of PCR-based diagnosis of Clostridioides difficile infections: Painful lessons from clinical trials.基于 PCR 的艰难梭菌感染诊断的风险：临床试验的惨痛教训。
Anaerobe. 2019 Dec;60:102048. doi: 10.1016/j.anaerobe.2019.06.001. Epub 2019 Jun 12.
6
Laboratory comparison between cell cytotoxicity neutralization assay and ultrasensitive single molecule counting technology for detection of Clostridioides difficile toxins A and B, PCR, enzyme immunoassays, and multistep algorithms.实验室比较细胞毒性中和测定法与超敏单分子计数技术，用于检测艰难梭菌毒素 A 和 B、PCR、酶免疫测定法和多步骤算法。
Diagn Microbiol Infect Dis. 2019 Sep;95(1):20-24. doi: 10.1016/j.diagmicrobio.2019.04.002. Epub 2019 Apr 9.
7
Cross-sectional study of the prevalence, causes and management of hospital-onset diarrhoea.医院获得性腹泻的患病率、病因及处理的横断面研究。
J Hosp Infect. 2019 Oct;103(2):200-209. doi: 10.1016/j.jhin.2019.05.001. Epub 2019 May 8.
8
Advances in Diagnostic Testing that Impact Infection Prevention and Antimicrobial Stewardship Programs.影响感染预防和抗菌药物管理计划的诊断检测进展。
Curr Infect Dis Rep. 2019 May 1;21(6):20. doi: 10.1007/s11908-019-0676-7.
9
Ultrasensitive Detection of Clostridium difficile Toxins Reveals Suboptimal Accuracy of Toxin Gene Cycle Thresholds for Toxin Predictions.超敏检测艰难梭菌毒素揭示毒素基因循环阈值对毒素预测的准确性欠佳。
J Clin Microbiol. 2019 May 24;57(6). doi: 10.1128/JCM.01885-18. Print 2019 Jun.
10
Global burden of infections: a systematic review and meta-analysis.全球 感染负担：系统评价和荟萃分析。
J Glob Health. 2019 Jun;9(1):010407. doi: 10.7189/jogh.09.010407.
Zotero 插件