Jégou S, Javoy-Agid F, Delbende C, Tranchand-Bunel D, Coy D H, Agid Y, Vaudry H
UA CNRS 650, Unité Alliée à l'INSERM Université de Rouen, Mont-Saint-Aignan, France.
Peptides. 1988 Jul-Aug;9(4):787-93. doi: 10.1016/0196-9781(88)90123-4.
The distribution of vasoactive intestinal peptide (VIP) in the post-mortem human brain was determined by radioimmunoassay using a highly specific antiserum. The detection limit of the assay was 4 fmol/tube. The highest concentrations of VIP were found in the cerebral cortex, amygdala, hypothalamus and hippocampus. The lowest levels of peptide were detected in basal ganglia including caudate nucleus, external pallidum, putamen and substantia nigra. All dilution curves of acetic acid extracts from different brain areas were strictly parallel to the standard curve. Sephadex G-50 gel filtration of frontal cortex extract showed that VIP-like immunoreactivity (VIP-LI) eluted as a major peak comigrating with synthetic hVIP. Detailed mapping of VIP in the human cerebral cortex showed the existence of a rostro-caudal gradient of VIP-LI concentrations: the frontal cortex exhibited the highest VIP levels, the parietal and temporal cortex contained medium values and the occipital cortex contained the lowest VIP levels. The concentrations of VIP-LI were compared in various regions of the human brain from normal and parkinsonian subjects. No significant changes in VIP-LI levels occurred in the brains of patients dying with Parkinson's disease. No difference in VIP levels could be found either when the parkinsonian group was subdivided into nondemented and demented patients. These data indicate that VIP-containing neurons are not affected in parkinsonian patients. Our results also suggest that VIP neuronal systems are not involved in the course of dementing process in Parkinson's disease.
使用高特异性抗血清，通过放射免疫分析法测定了尸检人脑内血管活性肠肽（VIP）的分布。该分析方法的检测限为4 fmol/管。在大脑皮质、杏仁核、下丘脑和海马中发现VIP浓度最高。在包括尾状核、外苍白球、壳核和黑质在内的基底神经节中检测到的肽水平最低。来自不同脑区的乙酸提取物的所有稀释曲线都与标准曲线严格平行。额叶皮质提取物的Sephadex G - 50凝胶过滤显示，VIP样免疫反应性（VIP - LI）作为与合成人VIP共迁移的主要峰洗脱。在人类大脑皮质中对VIP进行详细定位显示，存在VIP - LI浓度的前后梯度：额叶皮质VIP水平最高，顶叶和颞叶皮质含量中等，枕叶皮质VIP水平最低。比较了正常人和帕金森病患者人脑不同区域的VIP - LI浓度。死于帕金森病的患者大脑中VIP - LI水平没有显著变化。当将帕金森病组细分为非痴呆和痴呆患者时，也未发现VIP水平有差异。这些数据表明，帕金森病患者中含VIP的神经元未受影响。我们的结果还表明，VIP神经元系统不参与帕金森病的痴呆过程。
