Canciglia P, Martin J L, Bolis C L, Randall D, Magistretti P J
Instito di Fisiologia Generale, Università di Messina, Italy.
Biol Signals. 1995 Mar-Apr;4(2):86-93. doi: 10.1159/000109426.
The content of vasoactive intestinal peptide (VIP) was determined by radioimmunoassay in different brain regions of three fish species, namely salmon, trout and carp. The VIP extracted from salmon brain was shown by reverse-phase high-performance liquid chromatography to coelute with native porcine VIP. Highest VIP levels were measured in the salmon brain with a maximum of 112 pg/mg protein in the hypothalamus, followed by the telencephalon, olfactory bulb and optic tectum. In contrast, in the trout brain highest levels were found in the olfactory bulb (111 pg/mg protein). Lower levels were measured in the hypothalamus, telencephalon and cerebellum. The VIP content of the carp brain was considerably less, with highest values measured in the hypothalamus (12 pg/mg protein). The specificity in regional distribution further stresses the role of VIP in neurotransmission and indicates a function in neuroendocrine mechanisms and in the processing of olfactory stimuli.
采用放射免疫分析法测定了鲑鱼、鳟鱼和鲤鱼这三种鱼类不同脑区血管活性肠肽（VIP）的含量。反相高效液相色谱法显示，从鲑鱼脑中提取的VIP与天然猪VIP共洗脱。鲑鱼脑中VIP水平最高，下丘脑最高可达112 pg/mg蛋白质，其次是端脑、嗅球和视顶盖。相比之下，鳟鱼脑中嗅球的VIP水平最高（111 pg/mg蛋白质），下丘脑、端脑和小脑的水平较低。鲤鱼脑的VIP含量要少得多，下丘脑的含量最高（12 pg/mg蛋白质）。区域分布的特异性进一步强调了VIP在神经传递中的作用，并表明其在神经内分泌机制和嗅觉刺激处理中发挥作用。
