Huang Liquan, Chen Guiyuan, Hu Bo, Liang Shuxia, Chu Wenya, Chen Li
Nursing Faculty, School of Medicine, Jinhua Polytechnic Jinhua 321007, Zhejiang, P. R. China.
Department of Obstetrics and Gynecology, Jinhua People's Hospital Jinhua 321000, Zhejiang, P. R. China.
Int J Clin Exp Pathol. 2020 Mar 1;13(3):403-410. eCollection 2020.
Peripherally inserted central catheter (PICC) is being increasingly used in critical care settings. However, PICC is associated with various complications, particularly venous thrombosis. Our aim was to observe the effects of preventive application of low molecular weight heparin on venous thrombosis in a PICC model.
All rabbits were randomly divided into four groups: a control group, and low/medium/high concentration of low molecular weight heparin groups. All rabbits were injected prophylactically with normal saline or low molecular weight heparin once a day for 7 days. A PICC model was constructed. The pathologic changes of ear vein, anterior vena cava, and venous thrombosis were investigated using hematoxylin and eosin (H&E). Biochemical testing was performed including prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time (TT). Serum D-dimer (D2D) and fibrinogen (FG) levels were detected using Enzyme-linked immunosorbent assay (ELISA).
X-ray results showed that the PICC model was successfully constructed. H&E results showed that preventive application of low molecular weight heparin significantly ameliorated the pathologic damage to ear vein and anterior vena cava in the PICC model. Furthermore, we found that preventive application of low molecular weight heparin inhibited venous thrombosis in the model by H&E stain. Moreover, it significantly reduced serum FG and D2D levels in PICC model. Biochemical testing results showed that PT, APTT, and TT were significantly elevated in the PICC model.
Our findings revealed that preventive application of low molecular weight heparin significantly ameliorates venous thrombosis in a PICC model.
外周静脉穿刺中心静脉导管（PICC）在重症监护环境中的使用越来越多。然而，PICC与各种并发症相关，尤其是静脉血栓形成。我们的目的是观察预防性应用低分子量肝素对PICC模型中静脉血栓形成的影响。
将所有兔子随机分为四组：对照组、低/中/高浓度低分子量肝素组。所有兔子每天预防性注射生理盐水或低分子量肝素一次，共7天。构建PICC模型。使用苏木精和伊红（H&E）染色研究耳静脉、前腔静脉的病理变化以及静脉血栓形成情况。进行生化检测，包括凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）和凝血酶时间（TT）。采用酶联免疫吸附测定（ELISA）检测血清D-二聚体（D2D）和纤维蛋白原（FG）水平。
X射线结果显示PICC模型构建成功。H&E染色结果显示，预防性应用低分子量肝素可显著改善PICC模型中耳静脉和前腔静脉的病理损伤。此外，通过H&E染色我们发现预防性应用低分子量肝素可抑制模型中的静脉血栓形成。而且，它可显著降低PICC模型中的血清FG和D2D水平。生化检测结果显示，PICC模型中的PT、APTT和TT显著升高。
我们的研究结果表明，预防性应用低分子量肝素可显著改善PICC模型中的静脉血栓形成。
