Wilkinson Stuart, Smart Katherine A, James Sue, Cook David J
1Brewing Science Section, Division of Food Sciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD UK.
2SABMiller Plc, SABMiller House, Church Street West, Woking, Surrey GU21 6HS UK.
Bioenergy Res. 2017;10(1):146-157. doi: 10.1007/s12155-016-9782-7. Epub 2016 Aug 8.
Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system ( and NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, 1 t of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however, it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.
报道了使用整合生物加工（CBP）从啤酒糟（BSG）生产生物乙醇的情况。每个CBP系统由一种主要的丝状真菌物种和一种次要的酵母物种组成，丝状真菌物种分泌分解生物质所需的酶，酵母物种将释放出的糖发酵成乙醇。有趣的是，尽管研究了几种真菌组合，但发现清酒发酵系统（和NCYC479）产生的乙醇浓度最高（10天内乙醇浓度达37 g/L）。在此基础上，1吨BSG（干重）在此过程中使用36 hL水可产生94千克乙醇。对清酒发酵系统中BSG里所选碳水化合物降解（CAZy）基因表达进行的定量逆转录聚合酶链反应（QRT-PCR）分析表明，半纤维素首先被分解，其次是纤维素。CBP方法的一个缺点是乙醇生产率较低；然而，它所需的能量和水投入较低，因此值得进一步研究和优化。
