Sun Peng, Sun Limin, Cui Jia, Liu Lili, He Qing
Department of Oncology, The Second Hospital of Dalian Medical University Dalian, China.
Am J Transl Res. 2020 Mar 15;12(3):950-958. eCollection 2020.
The essential roles of long noncoding RNA (lncRNA) have been identified by emerging literature in the non-small cell lung cancer (NSCLC). However, the role of lncRNA hyaluronan synthase 2 antisense 1 (HAS2-AS1) in the NSCLC tumorigenesis is not clear. Here, we investigate the role and mechanism of HAS2-AS1 in the NSCLC tumorigenesis. In the NSCLC tissue and cells, HAS2-AS1 was found to be up-regulated, which, in turn, indicated the poor prognosis of NSCLC patients. Functional experiments illustrated that HAS2-AS1 promoted the proliferation, invasion and gefitinib chemotherapy resistance of NSCLC cells. In , HAS2-AS1 knockdown suppressed the tumor growth. Mechanically, HAS2-AS1 recruited the lysine-specific demethylase 1 (LSD1) to the EphB3 promoter region to inhibit its transcription. In conclusion, this finding elucidates the essential roles of HAS2-AS1 in the NSCLC tumorigenesis, providing a possible treatment strategy for the NSCLC.
长链非编码RNA（lncRNA）的重要作用已被非小细胞肺癌（NSCLC）领域不断涌现的文献所证实。然而，lncRNA透明质酸合酶2反义1（HAS2-AS1）在NSCLC肿瘤发生中的作用尚不清楚。在此，我们研究了HAS2-AS1在NSCLC肿瘤发生中的作用及机制。在NSCLC组织和细胞中，发现HAS2-AS1上调，这反过来表明NSCLC患者预后不良。功能实验表明，HAS2-AS1促进NSCLC细胞的增殖、侵袭和吉非替尼化疗耐药。此外，敲低HAS2-AS1可抑制肿瘤生长。机制上，HAS2-AS1招募赖氨酸特异性去甲基化酶1（LSD1）至EphB3启动子区域以抑制其转录。总之，这一发现阐明了HAS2-AS1在NSCLC肿瘤发生中的重要作用，为NSCLC提供了一种可能的治疗策略。
