Zeng Qinghuang, Lian Wenchang, Wang Guizhi, Qiu Manping, Lin Lingmu, Zeng Renhe
1Department of Paediatrics, The Affiliated Hospital (Group) of Putian University, Putian, 351100 Fujian China.
Department of Pediatric Neurological Rehabilitation, Putian Children's Hospital, Putian, 351100 Fujian China.
3 Biotech. 2020 May;10(5):192. doi: 10.1007/s13205-020-02167-8. Epub 2020 Apr 4.
Hypoxic-ischemic (HI) brain injury has a high occurrence rate of 1-4 per 1000 live births and is the leading cause of neurological disabilities. Despite the improvement in neonatal care, the effectiveness of current therapeutic strategies is limited, and thus, additional therapies with better results are of much needed. Pterostilbene is a stilbenoid possessing numerous preventive and therapeutic properties. The current study aimed to assess whether pterostilbene exerted protective effects in neonatal rats against experimentally induced ischemic brain injury. Pterostilbene was administered via oral gavage from postnatal day 3 to day 8. Rat pups that were seven-day-old were exposed to hypoxic-ischemic insult via ligation of the common carotid artery and hypoxic environment exposure. Pterostilbene treatment reduced neuronal loss and infarct volume. Pterostilbene administration regulated the NF-κB pathway, and the levels of inflammatory mediators (Nitric oxide, TNF-α, IL-1β, and IL-6) were reduced. HI-induced oxidative stress was significantly reduced by pterostilbene, as presented by decreased production of malondialdehyde and reactive oxygen species. Levels of glutathione were enhanced by pterostilbene. Pterostilbene regulated Nrf2/HO-1 and JNK expression and activated the PI3K/Akt-mTOR signals. These findings suggest that pterostilbene is a candidate compound for the treatment of neonatal HI.
缺氧缺血性（HI）脑损伤在每1000例活产中发生率高达1 - 4例，是导致神经残疾的主要原因。尽管新生儿护理有所改善，但目前治疗策略的效果有限，因此，急需效果更好的其他治疗方法。紫檀芪是一种具有多种预防和治疗特性的芪类化合物。本研究旨在评估紫檀芪对新生大鼠实验性缺血性脑损伤是否具有保护作用。从出生后第3天至第8天通过口服灌胃给予紫檀芪。对7日龄的幼鼠通过结扎颈总动脉和暴露于缺氧环境来诱导缺氧缺血性损伤。紫檀芪治疗减少了神经元损失和梗死体积。给予紫檀芪可调节NF-κB信号通路，并降低炎症介质（一氧化氮、TNF-α、IL-1β和IL-6）的水平。紫檀芪显著降低了HI诱导的氧化应激，表现为丙二醛和活性氧的产生减少。紫檀芪提高了谷胱甘肽水平。紫檀芪调节Nrf2/HO-1和JNK表达，并激活PI3K/Akt-mTOR信号。这些发现表明紫檀芪是治疗新生儿HI的候选化合物。
