CRMSB, UMR 5536, University of Bordeaux and CNRS, F-33000 Bordeaux, France.
I3M, Common Laboratory CNRS-Siemens, University of Poitiers and Poitiers University Hospital, F-86073 Poitiers, France.
Nutrients. 2022 Feb 12;14(4):773. doi: 10.3390/nu14040773.
Polyphenols are natural compounds with promising prophylactic and therapeutic applications. However, their methods of extraction, using organic solvents, may prove to be unsuitable for daily consumption or for certain medical indications. Here, we describe the neuroprotective effects of grape polyphenols extracted in an eco-sustainable manner in a rat model of neonatal hypoxia-ischemia (NHI). Polyphenols (resveratrol, pterostilben and viniferin) were obtained using a subcritical water extraction technology to avoid organic solvents and heavy metals associated with chemical synthesis processes. A resveratrol or a polyphenol cocktail were administered to pregnant females at a nutritional dose and different time windows, prior to induction of NHI in pups. Reduced brain edema and lesion volumes were observed in rat pups whose mothers were supplemented with polyphenols. Moreover, the preservation of motor and cognitive functions (including learning and memory) was evidenced in the same animals. Our results pave the way to the use of polyphenols to prevent brain lesions and their associated deficits that follow NHI, which is a major cause of neonatal death and disabilities.
多酚是具有广阔应用前景的天然化合物，具有预防和治疗作用。然而，这些化合物的提取方法需要使用有机溶剂，这可能不适合日常食用或某些医疗用途。在这里，我们描述了一种生态可持续的方法提取的葡萄多酚对新生鼠缺氧缺血（NHI）模型的神经保护作用。多酚（白藜芦醇、紫檀芪和芪酚）是通过亚临界水提取技术获得的，该技术可避免与化学合成过程相关的有机溶剂和重金属。在诱导幼仔发生 NHI 之前，将白藜芦醇或多酚混合物以营养剂量和不同的时间窗给予怀孕的雌性动物。结果发现，接受多酚补充的大鼠幼仔的脑水肿和病变体积减少。此外，还证明了这些动物的运动和认知功能（包括学习和记忆）得到了保存。我们的研究结果为使用多酚预防 NHI 后导致的脑损伤及其相关缺陷铺平了道路，NHI 是新生儿死亡和残疾的主要原因。
