结合碳酸磷灰石支架的脱落人乳牙干细胞在Wistar大鼠初始牙槽骨重塑过程中增强BMP-2、BMP-7表达并减弱MMP-8表达( ) 。
Exfoliated Human Deciduous Tooth Stem Cells Incorporating Carbonate Apatite Scaffold Enhance BMP-2, BMP-7 and Attenuate MMP-8 Expression During Initial Alveolar Bone Remodeling in Wistar Rats ( ).
作者信息
Prahasanti Chiquita, Nugraha Alexander Patera, Saskianti Tania, Suardita Ketut, Riawan Wibi, Ernawati Diah Savitri
机构信息
Department of Periodontology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
Department of Orthodontics, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
出版信息
Clin Cosmet Investig Dent. 2020 Mar 24;12:79-85. doi: 10.2147/CCIDE.S245678. eCollection 2020.
BACKGROUND
Post-tooth extraction socket preservation is necessary due to alveolar bone resorptive patterns through regenerative dentistry approaches that involve the use of stem cells, scaffold and growth factor. Stem cells derived from human exfoliated deciduous teeth (SHED) are known to potentially possess the osteogenic ability. Meanwhile, carbonate apatite scaffold (CAS) can act as a biocompatible scaffold capable of supporting mesenchymal stem cells (MSCs) to proliferate and differentiate optimally. The aim of this study is to investigate the expression of bone morphogenic protein-2 and 7 (BMP2, BMP7) and Matrix Metalloproteinase-8 (MMP-8) after the transplantation of SHED-incorporated CAS during in vivo bone remodeling.
MATERIAL AND METHODS
A total of 14 healthy, male, Wistar rats, whose mandible anterior teeth were extracted by means of sterile needle holder clamps, constituted the subjects of this study of alveolar bone defects. Two research groups were created: a control group (CAS) as group I and an experimental group (CAS + SHED) as group II. SHED with a density of 10 cells were incorporated into CAS before being transplanted into the experimental group. After 7 days, all the animals were sacrificed and their mandible anterior region extracted. The BMP2, BMP7 and MMP-8 expression were subsequently analyzed by means of immunostaining. An unpaired -test was conducted to analyze the treatment and control group (p<0.01) data.
RESULTS
The expression of BMP-2 and BMP-7 was higher in group II compared to group I. Meanwhile, the level of MMP-8 was lower in group II than group I. There was greater significant increased expression of BMP-2 and BMP-7 expression in Group II compared to Group I. There was significant decreased expression of MMP-8 between group II than group I (p<0.01).
CONCLUSION
SHED-incorporated CAS can enhance BMP-2 and BMP-7 expression while attenuating MMP-8 expression during in vivo alveolar bone remodeling.
背景
由于牙槽骨吸收模式，通过涉及使用干细胞、支架和生长因子的再生牙科方法进行拔牙后牙槽窝保存是必要的。已知源自人脱落乳牙的干细胞（SHED）可能具有成骨能力。同时，碳酸磷灰石支架（CAS）可作为一种生物相容性支架，能够支持间充质干细胞（MSC）最佳地增殖和分化。本研究的目的是调查在体内骨重塑过程中，将SHED与CAS联合移植后骨形态发生蛋白2和7（BMP2、BMP7）以及基质金属蛋白酶8（MMP - 8）的表达情况。
材料与方法
总共14只健康雄性Wistar大鼠，其下颌前牙通过无菌持针器夹拔除，构成了本牙槽骨缺损研究的对象。创建了两个研究组：作为第一组的对照组（CAS）和作为第二组的实验组（CAS + SHED）。将密度为10个细胞的SHED与CAS混合后再移植到实验组。7天后，处死所有动物并取出其下颌前部区域。随后通过免疫染色分析BMP2、BMP7和MMP - 8的表达。进行非配对t检验以分析治疗组和对照组（p<0.01）的数据。
结果
与第一组相比，第二组中BMP - 2和BMP - 7的表达更高。同时，第二组中MMP - 8的水平低于第一组。与第一组相比，第二组中BMP - 2和BMP - 7表达的增加更显著。第二组与第一组之间MMP - 8的表达显著降低（p<0.01）。
结论
在体内牙槽骨重塑过程中，将SHED与CAS联合使用可增强BMP - 2和BMP - 7的表达，同时减弱MMP - 8的表达。
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