Pediatric Dentistry Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia.
Orthodontics Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia.
F1000Res. 2020 Sep 22;9:1164. doi: 10.12688/f1000research.25009.2. eCollection 2020.
: Stem cells from human exfoliated deciduous teeth (SHED) seeded in carbonate apatite scaffold (CAS) may have multiple functions that could be used to regenerate the alveolar bone defects. The purpose of this study is to examine the ability of SHED and CAS in alveolar bone defects using an immunohistochemical analysis. : ten three-month-old healthy male Wistar rats ) that weighed between 150-250 grams (g) were used as animal models. A simple blind random sampling method was used to select the sample that was assigned to the study group for CAS and SHED seeded in CAS (n=5). The animal study model of the alveolar bone was established by extracting the anterior mandible teeth. Rodent anesthesia was applied to relieve the pain during the procedure for all test animals. Immunohistochemistry was performed after seven days to facilitate the examination of the receptor activator of NF-κβ ligand (RANKL), osteoprotegrin (OPG), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF), runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteocalcin, and osteopontin expression. The data was analyzed using the unpaired t-test (p<0.01) and Pearson's correlation test (p<0.05). : The OPG, RUNX2, TGF-β, VEGF, ALP, osteocalcin, and ostepontin expressions were higher in SHED seeded in CAS than CAS only with a significant difference between the groups (p<0.01). Furthermore, the RANKL expression was lower in SHED seeded in CAS compared to CAS only. There was a strong reverse significant correlation between OPG and RANKL expression (p<0.05). s: The number of osteogenic marker expressing cells, such as OPG, RUNX2, TGF-β, VEGF, ALP, osteocalcin, and ostepontin, increased. However, RANKL expression in the alveolar bone defects that were implanted with SHED seeded in CAS did not increase after seven days.
: 来源于人脱落乳牙的干细胞（SHED）种植在碳酸磷灰石支架（CAS）中可能具有多种功能，可用于再生牙槽骨缺损。本研究的目的是通过免疫组织化学分析来检验 SHED 和 CAS 在牙槽骨缺损中的作用。: 实验选用 10 只 3 月龄健康雄性 Wistar 大鼠（体重 150-250g）作为动物模型。采用简单盲法随机抽样方法，选取 CAS 中种植 SHED 组（n=5）的样本进行研究。通过提取前颌骨牙齿建立牙槽骨动物模型。对所有实验动物均采用啮齿动物麻醉以缓解手术过程中的疼痛。7 天后进行免疫组织化学检测，以方便检测核因子-κB 受体激活剂配体（RANKL）、骨保护素（OPG）、转化生长因子-β（TGF-β）、血管内皮生长因子（VEGF）、 runt 相关转录因子 2（RUNX2）、碱性磷酸酶（ALP）、骨钙素和骨桥蛋白的表达。采用独立样本 t 检验（p<0.01）和 Pearson 相关检验（p<0.05）进行数据分析。: SHED 种植在 CAS 中的 OPG、RUNX2、TGF-β、VEGF、ALP、骨钙素和骨桥蛋白表达均高于单纯 CAS，组间差异有统计学意义（p<0.01）。此外，SHED 种植在 CAS 中的 RANKL 表达低于单纯 CAS。OPG 和 RANKL 表达之间呈强负显著相关（p<0.05）。s：表达成骨标记物的细胞数量增加，如 OPG、RUNX2、TGF-β、VEGF、ALP、骨钙素和骨桥蛋白。然而，植入 SHED 种植在 CAS 中的牙槽骨缺损中 RANKL 表达在 7 天后没有增加。
