Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Biosens Bioelectron. 2020 Jun 15;158:112179. doi: 10.1016/j.bios.2020.112179. Epub 2020 Mar 27.
Herein, an innovative photocathodic enzymatic biosensor is proposed with poly {4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]-benzo[1,2-b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophene-4,6-diyl} (PTB7-Th) as donor-acceptor-type photoactive material and three-dimensional (3D) polyaniline hydrogels (PAniHs) as both electron transfer layer and biomolecule carrier. Based on the enhancement effect of PAniHs on the charge separation and electron transfer of PTB7-Th and the competitive consumption of dissolved oxygen (O) between the xanthine oxidase (XOD)-guanine catalytic reaction and O-sensitive PTB7-Th/PAniHs, the proposed photocathodic enzymatic biosensor has been demonstrated to detect guanine with the advantages of low limit of detection (0.02 μM), wide linear range (from 0.1 to 80 μM), simple and convenient preparation process, satisfactory stability, and photochemical signal amplification independent of any exogenous electron donor/acceptor or sensitizer. Remarkably, the proposed photocathodic enzymatic biosensor can not only be extended to other aerobic enzymatic bioanalyses, but also pave a horizon for the application of environmentally friendly conductive hydrogel materials in photoelectrochemical bioanalysis.
本文提出了一种创新性的光阴极酶生物传感器，以聚{4,8-双[5-(2-乙基己基)噻吩-2-基]苯并[1,2-b:4,5-b']二噻吩-2,6-二基--alt-3-氟-2-[(2-乙基己基)羰基]噻吩[3,4-b]噻吩-4,6-二基}（PTB7-Th）作为供体-受体型光活性材料，三维（3D）聚苯胺水凝胶（PAniHs）作为电子传输层和生物分子载体。基于 PAniHs 对 PTB7-Th 电荷分离和电子转移的增强作用以及黄嘌呤氧化酶（XOD）-鸟嘌呤催化反应与 O 敏感的 PTB7-Th/PAniHs 之间溶解氧（O）的竞争消耗，所提出的光阴极酶生物传感器已被证明可用于检测鸟嘌呤，具有低检测限（0.02 μM）、宽线性范围（从 0.1 到 80 μM）、简单方便的制备工艺、令人满意的稳定性以及独立于任何外源电子供体/受体或敏化剂的光电化学信号放大等优点。值得注意的是，所提出的光阴极酶生物传感器不仅可以扩展到其他需氧酶生物分析，而且为环保导电水凝胶材料在光电化学生物分析中的应用开辟了道路。
