Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, Johannesburg, South Africa.
Molecules. 2020 Jun 24;25(12):2900. doi: 10.3390/molecules25122900.
Cancer, pain and inflammation have long been a cause for concern amongst patients, clinicians and research scientists. There is an alarming increase in the demand for medicines suppressing these disease conditions. The present study investigates the role of bark methanol (SMBM) extract against MCF-7 breast cancer cells, pain and inflammation. The MCF-7 cells treated with SMBM were analyzed for adenosine triphosphate (ATP), lactate dehydrogenase (LDH) levels, changes in cell morphology and nuclear damage. Hot plate, acetic acid and formalin-induced pain models were followed to determine the analgesic activity. Anti-inflammatory activity was studied using carrageenan, egg albumin and cotton pellet induced rat models. Microscopic images of cells in SMBM treated groups showed prominent cell shrinkage and nuclear damage. Hoechst stain results supported the cell death morphology. The decline in ATP (47.96%) and increased LDH (40.96%) content indicated SMBM induced toxicity in MCF-7 cells. In the in vivo study, a higher dose (200 mg/kg) of the extract was found to be effective in reducing pain and inflammation. The results are promising and the action of the extract on MCF-7 cells, pain and inflammation models indicate the potential of drugs of natural origin to improve current therapies.
癌症、疼痛和炎症一直是患者、临床医生和研究科学家关注的问题。抑制这些疾病状况的药物的需求呈惊人的增长趋势。本研究调查了树皮甲醇（SMBM）提取物对 MCF-7 乳腺癌细胞、疼痛和炎症的作用。用 SMBM 处理的 MCF-7 细胞分析了三磷酸腺苷（ATP）、乳酸脱氢酶（LDH）水平、细胞形态变化和核损伤。采用热板、醋酸和甲醛诱导疼痛模型来确定镇痛活性。采用角叉菜胶、卵白蛋白和棉塞诱导的大鼠模型研究抗炎活性。在 SMBM 处理组的细胞的显微镜图像显示出明显的细胞收缩和核损伤。Hoechst 染色结果支持细胞死亡形态。ATP（47.96%）的下降和 LDH（40.96%）含量的增加表明 SMBM 诱导 MCF-7 细胞毒性。在体内研究中，发现较高剂量（200mg/kg）的提取物可有效减轻疼痛和炎症。结果很有希望，提取物对 MCF-7 细胞、疼痛和炎症模型的作用表明天然药物来源的药物有潜力改善当前的治疗方法。
