School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, People's Republic of China.
Graduate School, Jilin Institute of Chemical Technology, Jilin 132022, People's Republic of China.
J Agric Food Chem. 2020 Apr 29;68(17):4893-4902. doi: 10.1021/acs.jafc.0c01473. Epub 2020 Apr 21.
Ginsenoside Rg3 (GRg3) is one of the major bioactive ingredients of ginseng, which is not only used as a herbal medicine but also used as a functional food to support body functions. In this study, the beneficial effects of GRg3 on rheumatoid arthritis (RA) mice was evaluated from anti-inflammatory and immunosuppressive aspects. The footpad swelling rate, pathological changes of the ankle joint, and levels of tumor necrosis factor α, interleukin 6, interleukin 10, and tumor necrosis factor β were used to assess the anti-inflammatory effect of GRg3 on RA mice. Flow cytometric analysis of CD4CD25Foxp3Treg cell percentage and metabolomic analysis based on gas chromatography-tandem mass spectrometry were used to assess the immunosuppressive effect and underlying mechanisms. GRg3 exhibited anti-inflammatory and immunosuppressive effects on RA mice. The potential mechanisms were related to regulate the pathways of oxidative phosphorylation and enhance the function of CD4CD25Foxp3Treg cells to maintain peripheral immune tolerance of RA mice. These findings can provide a preliminary experimental basis to exploit GRg3 as a functional food or an effective complementary for the adjuvant therapy of RA.
人参皂苷 Rg3（GRg3）是人参的主要生物活性成分之一，不仅被用作草药，还被用作功能性食品来支持身体功能。在这项研究中，从抗炎和免疫抑制方面评估了 GRg3 对类风湿性关节炎（RA）小鼠的有益作用。爪垫肿胀率、踝关节病理变化以及肿瘤坏死因子 α、白细胞介素 6、白细胞介素 10 和肿瘤坏死因子 β 的水平用于评估 GRg3 对 RA 小鼠的抗炎作用。基于气相色谱-串联质谱的流式细胞术分析 CD4CD25Foxp3Treg 细胞的百分比和代谢组学分析用于评估免疫抑制作用及其潜在机制。GRg3 对 RA 小鼠具有抗炎和免疫抑制作用。潜在的机制与调节氧化磷酸化途径和增强 CD4CD25Foxp3Treg 细胞的功能有关，以维持 RA 小鼠的外周免疫耐受。这些发现可以为开发 GRg3 作为功能性食品或作为 RA 辅助治疗的有效补充提供初步的实验基础。
