万古霉素中介耐药金黄色葡萄球菌毒力组学和耐药组学研究。
Study on the virulome and resistome of a vancomycin intermediate-resistance Staphylococcus aureus.
机构信息
Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, 510623, China.
Research Center for Translational Medicine, The Second Affiliated Hospital, Medical College of Shantou University, Shantou 515041, China.
出版信息
Microb Pathog. 2020 Aug;145:104187. doi: 10.1016/j.micpath.2020.104187. Epub 2020 Apr 7.
Methicillin-resistant S. aureus (MRSA) has been considered a potential "Super Bugs", responsible for various infectious diseases. Vancomycin has been the most effective antibitic to treat MRSA originated infections. In this study, we aimed at investigating the genomic features of a vancomycin intermediate-resistance S. aureus strain Guangzhou-SauVS2 isolated from a female patient suffering from chronic renal function failure, emphasizing on its antimicrobial resistance and virulence determinants. The genome has a total length of 2,605,384 bp and the G+C content of 33.21%, with 2,239 predicted genes annotated with GO terms, COG categories, and KEGG pathways. Besides the carriage of vancomycin b-type resistance protein responsible for the vancomycin intermediate-resistance, S. aureus strain Guangzhou-SauVS2 showed resistance to β-lactams, quinolones, macrolide, and tetracycline, due to the acquisition of corresponding antimicrobial resistance genes. In addition, virulence factors including adherence, antiphagocytosis, iron uptake, and toxin were determined, indicating the pathogenesis of the strain.
耐甲氧西林金黄色葡萄球菌（MRSA）被认为是一种潜在的“超级细菌”，可导致多种传染病。万古霉素一直是治疗耐甲氧西林金黄色葡萄球菌感染的最有效抗生素。在这项研究中，我们旨在研究从一名患有慢性肾功能衰竭的女性患者中分离出的万古霉素中介耐药金黄色葡萄球菌菌株 Guangzhou-SauVS2 的基因组特征，重点研究其抗菌药物耐药性和毒力决定因素。该基因组全长 2,605,384bp，G+C 含量为 33.21%，有 2,239 个预测基因被注释为 GO 术语、COG 类别和 KEGG 途径。除了携带负责万古霉素中介耐药的万古霉素 b 型耐药蛋白外，金黄色葡萄球菌菌株 Guangzhou-SauVS2 还因获得相应的抗菌药物耐药基因而对β-内酰胺类、喹诺酮类、大环内酯类和四环素类药物产生耐药性。此外，还确定了粘附、抗吞噬、铁摄取和毒素等毒力因子，表明该菌株的发病机制。
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