Faculty of Food Science and Engineering, Dunărea de Jos University of Galati, Romania, 111, Domnească Street, 800201 Galati, Romania.
Molecules. 2020 Apr 8;25(7):1700. doi: 10.3390/molecules25071700.
Anthocyanins from black currant extract and lactic acid bacteria were co-microencapsulated using a gastro-intestinal-resistant biocomposite of whey protein isolate, inulin, and chitosan, with an encapsulation efficiency of 95.46% ± 1.30% and 87.38% ± 0.48%, respectively. The applied freeze-drying allowed a dark purple stable powder to be obtained, with a satisfactory content of phytochemicals and 11 log colony forming units (CFU)/g dry weight of powder (DW). Confocal laser microscopy displayed a complex system, with several large formations and smaller aggregates inside, consisting of biologically active compounds, lactic acid bacteria cells, and biopolymers. The powder showed good storage stability, with no significant changes in phytochemicals and viable cells over 3 months. An antioxidant activity of 63.64 ± 0.75 mMol Trolox/g DW and an inhibitory effect on α-amylase and α-glucosidase of 87.10% ± 2.08% and 36.96% ± 3.98%, respectively, highlighted the potential biological activities of the co-microencapsulated powder. Significantly, the in vitro digestibility profile showed remarkable protection in the gastric environment, with controlled release in the intestinal simulated environment. The powder was tested by addition into a complex food matrix (yogurt), and the results showed satisfactory stability of biologically active compounds when stored for 21 d at 4 °C. The obtained results confirm the important role of microencapsulation in ensuring a high degree of protection, thus allowing new approaches in developing food ingredients and nutraceuticals, with enhanced functionalities.
黑加仑提取物中的花色苷和乳酸菌被共同包埋在乳清分离蛋白、菊粉和壳聚糖组成的胃肠耐抗性生物复合材料中，包埋效率分别为 95.46%±1.30%和 87.38%±0.48%。应用冷冻干燥得到了深紫色的稳定粉末，具有令人满意的植物化学物质含量和 11 对数菌落形成单位（CFU）/g 干重（DW）的活菌数。共聚焦激光显微镜显示出一个复杂的系统，其中有几个大的形成物和内部较小的聚集物，由生物活性化合物、乳酸菌细胞和生物聚合物组成。该粉末具有良好的储存稳定性，在 3 个月内植物化学物质和活菌数没有明显变化。抗氧化活性为 63.64±0.75mMol Trolox/g DW，对α-淀粉酶和α-葡萄糖苷酶的抑制率分别为 87.10%±2.08%和 36.96%±3.98%，突出了共微囊化粉末的潜在生物活性。值得注意的是，体外消化率曲线显示出在胃环境中具有显著的保护作用，在肠道模拟环境中具有控制释放的特性。该粉末被添加到复杂的食品基质（酸奶）中进行测试，结果表明，在 4°C 下储存 21 天时，生物活性化合物具有令人满意的稳定性。所得结果证实了微囊化在确保高度保护方面的重要作用，从而为开发具有增强功能的食品配料和营养保健品提供了新的方法。
