School of Biochemistry, University of Bristol, Bristol, UK.
Bristol Centre for Functional Nanomaterials, HH Wills Physics Laboratory, University of Bristol, Bristol, UK.
Biotechnol Appl Biochem. 2020 Jul;67(4):527-535. doi: 10.1002/bab.1924. Epub 2020 May 22.
The ability to perform organic reactions with chemoselectivity is of critical importance in synthesis. Recently, we reported that a de novo carbene transferase, a tetra-α-helical c-type heme-containing protein, C45, is proficient at N-H insertion reactions, proceeding via the intermolecular transfer of a metallocarbenoid intermediate into the N-H σ-bond to form a new N-C σ-bond. Here we demonstrate that C45 can also catalyse N-H insertion reactions chemoselectively, even when the substrate contains an unprotected hydroxyl group.
在合成中，具有化学选择性地进行有机反应的能力至关重要。最近，我们报道了一种从头开始的碳烯转移酶，即一种四α-螺旋 c 型含血红素蛋白 C45，擅长进行 N-H 插入反应，通过金属碳烯中间体的分子间转移到 N-H σ 键中形成新的 N-C σ 键。在这里，我们证明 C45 即使在底物含有未保护的羟基时，也可以选择性地催化 N-H 插入反应。
