Siddique I H, Mohamed A A
Department of Microbiology, School of Veterinary Medicine, Tuskegee University, Alabama 36088.
Acta Microbiol Hung. 1988;35(3):283-7.
Cell-mediated immunity to Leptospira pomona was measured in goats by the leukocyte-migration inhibition test (LMIT) and microassay for stimulation of protein synthesis (SPS) with 3H-leucine. L. pomona soluble antigen was used in both tests. The migration indices in the group of goats infected with L. pomona were significantly lower (less than .001) as compared to control. The migration zones of the unstimulated peripheral blood leukocytes in the infected group were significantly smaller (less than .001) than those of the control. The SPS test was strongly positive in test animals and was negative in control.
通过白细胞迁移抑制试验（LMIT）和用³H-亮氨酸刺激蛋白质合成的微量测定法（SPS），在山羊中测定了对波摩那群钩端螺旋体的细胞介导免疫。两种试验均使用波摩那群钩端螺旋体可溶性抗原。与对照组相比，感染波摩那群钩端螺旋体的山羊组的迁移指数显著更低（小于0.001）。感染组未受刺激的外周血白细胞的迁移区明显小于对照组（小于0.001）。SPS试验在试验动物中呈强阳性，而在对照组中呈阴性。
