Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, United States.
Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, United States.
Methods. 2021 Jun;190:72-79. doi: 10.1016/j.ymeth.2020.04.003. Epub 2020 Apr 8.
Lymphatic vessels, as a means to metastasize, are frequently recruited by tumor tissues during their progression. However, reliable in vitro models to dissect the intricate crosstalk between lymphatic vessels and tumors are still in urgent demand. Here, we describe a tissue-engineering method based on sacrificial bioprinting, to develop an enabling model of the human breast tumor with embedded multiscale lymphatic vessels, which is compatible with existing microscopy to examine the processes of lymphatic vessel sprouting and breast tumor cell migration in a physiologically relevant volumetric microenvironment. This platform will potentially help shed light on the complex biology of the tumor microenvironment, tumor lymphangiogenesis, lymphatic metastasis, as well as tumor anti-lymphangiogenic therapy in the future. We further anticipate wide adoption of the method to the production of various tissues and their models with incorporation of lymphatics vessels towards relevant applications.
淋巴管作为转移的途径，在肿瘤进展过程中经常被肿瘤组织招募。然而，能够解析淋巴管和肿瘤之间复杂串扰的可靠体外模型仍然迫切需要。在这里，我们描述了一种基于牺牲性生物打印的组织工程方法，来开发一种具有嵌入式多尺度淋巴管的人乳腺癌肿瘤模型，该模型与现有的显微镜兼容，可在生理相关的体积微环境中检查淋巴管发芽和乳腺癌细胞迁移的过程。该平台将有助于揭示肿瘤微环境、肿瘤淋巴管生成、淋巴转移以及肿瘤抗淋巴管生成治疗的复杂生物学，我们还预计该方法将广泛应用于各种组织及其带有淋巴管的模型的生产，以满足相关应用的需求。
