Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Fish Shellfish Immunol. 2020 Aug;103:37-46. doi: 10.1016/j.fsi.2020.04.003. Epub 2020 Apr 8.
Turbot (Scophthalmus maximus) is an economically important marine fish cultured in China. In this study, fish in the experimental group were exposed to four temperatures: 15, 20, 25 and 28 °C. Metabolomics analysis and quantitative real-time PCR were used to assess changes in metabolic profiling and gene expression associated with thermal stress. The results showed the levels of heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), blood creatinine and cortisol in S. maximus were all significantly upregulated (P < 0.05), indicating a stress response at 25 °C or higher. Challenge with thermal stress significantly increased expression levels of succinate dehydrogenase (SDH), fructose-1, 6-bisphosphatase (FBPase), malate dehydrogenase (MDH), cytosolic phosphoenolpyruvate carboxykinase (cPEPCK), glucose-6-phosphatase (G6Pase) and aspartate aminotransferase (AST) (P < 0.05). However, there was no effect on the expression levels of lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and mitochondrial phosphoenolpyruvate carboxykinase (mPEPCK). Moreover, high temperature decreased levels of glycogenic amino acids, including histidine, threonine, glutamine, phenylalanine, arginine, serine, tyrosine, methionine and isoleucine. These findings suggest a significant correlation between gene expression and regulation of carbohydrate and amino acid metabolism in heat-stressed S. maximus kidney. In addition, the maintenance of aerobic metabolism and activation of gluconeogenesis appeared to be a critical metabolic strategy in combating heat stress in turbot kidney.
大菱鲆（Scophthalmus maximus）是中国重要的海水养殖鱼类之一。本研究中，实验组鱼分别暴露于 15、20、25 和 28°C 四种温度下。采用代谢组学分析和实时定量 PCR 技术，评估了与热应激相关的代谢谱和基因表达变化。结果表明，热休克蛋白 70（HSP70）、热休克蛋白 90（HSP90）、血液肌酸酐和皮质醇水平在 25°C 或更高温度下均显著上调（P<0.05），表明存在应激反应。热应激挑战显著增加了琥珀酸脱氢酶（SDH）、果糖-1,6-二磷酸酶（FBPase）、苹果酸脱氢酶（MDH）、细胞质磷酸烯醇丙酮酸羧激酶（cPEPCK）、葡萄糖-6-磷酸酶（G6Pase）和天冬氨酸氨基转移酶（AST）的表达水平（P<0.05）。然而，对乳酸脱氢酶（LDH）、丙氨酸氨基转移酶（ALT）和线粒体磷酸烯醇丙酮酸羧激酶（mPEPCK）的表达水平没有影响。此外，高温降低了糖质氨基酸的水平，包括组氨酸、苏氨酸、谷氨酸、苯丙氨酸、精氨酸、丝氨酸、酪氨酸、蛋氨酸和异亮氨酸。这些发现表明，在热应激大菱鲆肾脏中，基因表达与碳水化合物和氨基酸代谢的调节之间存在显著相关性。此外，有氧代谢的维持和糖异生的激活似乎是大菱鲆肾脏抵抗热应激的关键代谢策略。
