Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, 266071, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China.
J Therm Biol. 2022 Feb;104:103141. doi: 10.1016/j.jtherbio.2021.103141. Epub 2021 Dec 27.
The hearts of fish play a major role in their physiological plasticity and acclimation to different thermal conditions. To understand the precise mechanism and the pathways activated by thermal cardiac stress in fish, we sampled cardiac tissue from juvenile turbot (Scophthalmus maximus) exposed to control (14°C) and test (20°C, 24°C, and 28°C) conditions, and performed digital RNA sequencing (RNA-seq). A total of 3359 differentially expressed genes (DEGs) were identified. The results of an expression tendency analysis and KEGG annotation analysis of the DEGs demonstrated that energy metabolism played a core role in thermal stress in turbot for the majority of the up-regulated genes. This was followed by lipid metabolism, mitochondrial function, glycolysis, and carbohydrate metabolism. RNA modifications are gaining the interest of biologists worldwide. In this study, at the transcriptome level, our results showed that 246 m6A-containing genes were detected in the DEGs, which were related to EIF3C, EIF3D, EIF3J, METTL16, RBM15B, VIRMA, and YTHDC1. This indicates that mA is involved in the regulation of heat stress in turbot. This study is an important step towards understanding the cardiac adaptive response to thermal stress. Importantly, the plasticity of cardiac tissue could predict the adaptability of fish species to environmental temperature.
鱼类的心脏在其生理可塑性和对不同热条件的适应中起着重要作用。为了了解鱼类热心脏应激激活的精确机制和途径，我们从暴露于对照（14°C）和测试（20°C、24°C 和 28°C）条件下的幼大菱鲆（Scophthalmus maximus）心脏组织中取样，并进行了数字 RNA 测序（RNA-seq）。共鉴定出 3359 个差异表达基因（DEGs）。DEGs 的表达趋势分析和 KEGG 注释分析结果表明，在大多数上调基因中，能量代谢在大菱鲆的热应激中起着核心作用。其次是脂质代谢、线粒体功能、糖酵解和碳水化合物代谢。RNA 修饰正在引起全球生物学家的兴趣。在这项研究中，在转录组水平上，我们的结果表明，在 DEGs 中检测到 246 个含有 m6A 的基因，这些基因与 EIF3C、EIF3D、EIF3J、METTL16、RBM15B、VIRMA 和 YTHDC1 有关。这表明 mA 参与了大菱鲆热应激的调节。这项研究是理解心脏对热应激的适应反应的重要一步。重要的是，心脏组织的可塑性可以预测鱼类对环境温度的适应性。
