基于 EuNPs-mAb 荧光探针的免疫层析试纸条用于快速灵敏检测猪流行性腹泻病毒。

EuNPs-mAb fluorescent probe based immunochromatographic strip for rapid and sensitive detection of porcine epidemic diarrhea virus.

机构信息

Department of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.

出版信息

Talanta. 2020 Jul 1;214:120865. doi: 10.1016/j.talanta.2020.120865. Epub 2020 Feb 25.

Porcine epidemic diarrhea (PED), induced by porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration and high mortality in neonatal piglets, resulting in significant economic losses in the pig industries. In this study, an immunochromatographic assay (ICA) based on a EuNPs-mAb fluorescent probe was developed and optimized for rapid detection of PEDV. The limit of detection (LOD) of the ICA was 0.218 μg/mL (2.725 × 10 TCID/mL) and its linear detection range was 0.03125-8 μg/mL (3.91 × 10-10 TCID/mL). The ICA was also validated for the detection of PEDV in swine stool samples. 60 swine stool samples from southern China were analyzed by the ICA and RT-PCR, and the results showed that the coincidence rate of the ICA to RT-PCR was 86.67%, which was significantly higher than that of AuNPs based ICA. The ICA is sensitive and specific and can achieve on-site rapid detection of swine stool samples. Therefore, the ICA has a great potential for PED diagnosis and prevention.

猪流行性腹泻（PED）由猪流行性腹泻病毒（PEDV）引起，导致仔猪发生急性腹泻、呕吐、脱水和高死亡率，给养猪业造成重大经济损失。本研究基于 EuNPs-mAb 荧光探针建立并优化了一种免疫层析分析（ICA）方法，用于快速检测 PEDV。ICA 的检测限（LOD）为 0.218μg/mL（2.725×10 TCID/mL），线性检测范围为 0.03125-8μg/mL（3.91×10-10 TCID/mL）。该 ICA 还用于检测猪粪便中的 PEDV。用 ICA 和 RT-PCR 分析了来自中国南方的 60 个猪粪便样本，结果表明，ICA 与 RT-PCR 的符合率为 86.67%，明显高于基于金纳米颗粒（AuNPs）的 ICA。该 ICA 具有良好的敏感性和特异性，可实现现场快速检测猪粪便样本。因此，ICA 具有很大的潜力用于 PED 的诊断和预防。