School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China.
School of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China.
J Pharmacol Sci. 2020 Jul;143(3):156-164. doi: 10.1016/j.jphs.2020.03.005. Epub 2020 Mar 23.
Safranal (SFR) is the major constituent of saffron. The purpose of this study was to observe the effect of SFR on myocardial ischemia induced by isoprenaline (ISO) and to explore its possible mechanism. The myocardial ischemia rat model was established by subcutaneous injection of ISO (85 mg/kg/d) on the 8th and 9th day of the experiment. Serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured, as were changes in calcium concentration, reactive oxygen species (ROS) and cardiac morphology of the myocardial tissue. The effects of SFR on cell contraction, Ca transient and L-type Ca current (I) in isolated rat myocardial cells were measured using the Ion Optix detection system and the whole-cell patch-clamp technique. SFR can decrease the activity of serum CK, LDH and MDA, and increase the activity of serum SOD, reduce intracellular calcium concentration and the manufacture of ROS. In addition, SFR can improve changes in heart morphology. SFR can significantly inhibit contraction, Ca transients and I in isolated ventricular myocytes. SFR has a cardioprotective role in ISO-induced MI rats, and the underling mechanism is related to the inhibition of oxidative stress, myocardial contractility, I and the regulation of Ca homeostasis.
西红花中的主要成分是西红花醛（SFR）。本研究旨在观察 SFR 对异丙肾上腺素（ISO）诱导的心肌缺血的影响，并探讨其可能的机制。通过在实验的第 8 天和第 9 天皮下注射 ISO（85mg/kg/d）建立心肌缺血大鼠模型。测定血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、丙二醛（MDA）和超氧化物歧化酶（SOD）的变化，观察心肌组织钙浓度、活性氧（ROS）和心脏形态的变化。采用 Ion Optix 检测系统和全细胞膜片钳技术，测定 SFR 对分离大鼠心肌细胞收缩、Ca 瞬变和 L 型 Ca 电流（I）的影响。SFR 可降低血清 CK、LDH 和 MDA 的活性，提高血清 SOD 的活性，降低细胞内钙浓度和 ROS 的产生。此外，SFR 可以改善心脏形态的变化。SFR 可显著抑制分离心室肌细胞的收缩、Ca 瞬变和 I。SFR 对 ISO 诱导的 MI 大鼠具有心脏保护作用，其作用机制与抑制氧化应激、心肌收缩性、I 和钙稳态调节有关。
